74920 20200117221645.0 doi 10.1042/BCJ20180172 sideral 107715 ART-2018-107715 eng Raimi, O.G. Evidence for substrate-assisted catalysis in N-acetylphosphoglucosamine mutase 2018 Access copy available to the general public Unrestricted N-acetylphosphoglucosamine mutase (AGM1) is a key component of the hexosamine biosynthetic pathway that produces UDP-GlcNAc, an essential precursor for a wide range of glycans in eukaryotes. AGM belongs to the a-D-phosphohexomutase metalloenzyme superfamily and catalyzes the interconversion of N-acetylglucosamine-6-phosphate (GlcNAc-6P) to N-acetylglucosamine-1-phosphate (GlcNAc-1P) through N-acetylglucosa-mine-1, 6-bisphosphate (GlcNAc-1, 6-bisP) as the catalytic intermediate. Although there is an understanding of the phosphoserine-dependent catalytic mechanism at enzymatic and structural level, the identity of the requisite catalytic base in AGM1/phosphoglucomu-tases is as yet unknown. Here, we present crystal structures of a Michaelis complex of AGM1 with GlcNAc-6P and Mg2+, and a complex of the inactive Ser69Ala mutant together with glucose-1, 6-bisphosphate (Glc-1, 6-bisP) that represents key snapshots along the reaction co-ordinate. Together with mutagenesis, these structures reveal that the phosphate group of the hexose-1, 6-bisP intermediate may act as the catalytic base. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.331 2018 BIOCHEMISTRY & MOLECULAR BIOLOGY 72 / 294 = 0.245 2018 Q1 T1 2.142 2018 Biochemistry 2018 Q1 Molecular Biology 2018 Q1 Cell Biology 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-3122-9401 Hurtado-Guerrero, R. Universidad de Zaragoza Van Aalten, D.M.F. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 475, 15 (2018), 2547-2557 Biochem. j. BIOCHEMICAL JOURNAL 0264-6021 569349 http://zaguan.unizar.es/record/74920/files/texto_completo.pdf Versión publicada 52632 http://zaguan.unizar.es/record/74920/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:74920 articulos driver 2020-01-17-22:06:19 ARTICLE