000074949 001__ 74949
000074949 005__ 20200117221609.0
000074949 0247_ $$2doi$$a10.1186/s13071-018-3046-7
000074949 0248_ $$2sideral$$a107722
000074949 037__ $$aART-2018-107722
000074949 041__ $$aeng
000074949 100__ $$0(orcid)0000-0001-8198-8118$$aRuiz-Arrondo, I.
000074949 245__ $$aDNA barcoding of blackflies (Diptera: Simuliidae) as a tool for species identification and detection of hidden diversity in the eastern regions of Spain
000074949 260__ $$c2018
000074949 5060_ $$aAccess copy available to the general public$$fUnrestricted
000074949 5203_ $$aBackground: Blackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately. Methods: Blackflies specimens (larvae, pupae, reared adults and biting females) were collected in the period 2015-2017 in and near rivers and streams from different regions in Spain. A modified Hotshot technique was used for the DNA extraction and the cox1 DNA barcoding region of the cytochrome c oxidase subunit 1 was sequenced from the specimens collected. Results: In total, we collected 239 specimens representing 22 species. Of these, six species are new records for the Aragón region: P. tomosvaryi, S. bertrandi, S. galloprovinciale, S. lineatum, S. rubzovianum and S. xanthinum. Cox1 DNA barcode sequences for 21 species were recovered, including four species of the genus Prosimulium and 17 species of the genus Simulium [Boophthora (1 species), Eusimulium (1 species), Nevermannia (4 species), Simulium (s.s.) (6 species), Trichodagmia (1 species) and Wilhelmia (4 species)]. For the first time the complete DNA barcodes for five species (P. tomosvaryi, S. carthusiense, S. brevidens, S. monticola and S. sergenti) were registered. Most of the specimens belonging to the same recognized species were clustered together in the neighbour-joining tree, except for S. argyreatum, S. monticola and S. variegatum. The overall genetic distance in the dataset was 0.14%. The average of the intraspecific genetic divergence within the different taxa was 1.47% (0.05-3.96%). In contrast, the interspecific divergence varied between 2.50-22.0%. Conclusions: In this study we assessed the use of the cox1 DNA barcoding region for the identification of species of blackflies in Spain. Our results showed that combining DNA barcoding with morphology enhanced our taxonomic rationale in identifying the blackflies in the country.
000074949 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000074949 590__ $$a3.031$$b2018
000074949 591__ $$aTROPICAL MEDICINE$$b2 / 21 = 0.095$$c2018$$dQ1$$eT1
000074949 591__ $$aPARASITOLOGY$$b7 / 36 = 0.194$$c2018$$dQ1$$eT1
000074949 592__ $$a1.565$$b2018
000074949 593__ $$aParasitology$$c2018$$dQ1
000074949 593__ $$aInfectious Diseases$$c2018$$dQ1
000074949 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000074949 700__ $$aHernández-Triana, L.M.
000074949 700__ $$aIgnjatovic-Cupina, A.
000074949 700__ $$aNikolova, N.
000074949 700__ $$aGarza-Hernández, J.A.
000074949 700__ $$aRodríguez-Pérez, M.A.
000074949 700__ $$aOteo, J.A.
000074949 700__ $$aFooks, A.R.
000074949 700__ $$0(orcid)0000-0003-0663-8411$$aLucientes Curdi, J.$$uUniversidad de Zaragoza
000074949 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000074949 773__ $$g11, 1 (2018), 463 [7 pp]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000074949 8564_ $$s1873280$$uhttps://zaguan.unizar.es/record/74949/files/texto_completo.pdf$$yVersión publicada
000074949 8564_ $$s92703$$uhttps://zaguan.unizar.es/record/74949/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000074949 909CO $$ooai:zaguan.unizar.es:74949$$particulos$$pdriver
000074949 951__ $$a2020-01-17-21:47:54
000074949 980__ $$aARTICLE