000074953 001__ 74953 000074953 005__ 20200813075838.0 000074953 0247_ $$2doi$$a10.1038/s41598-018-28748-5 000074953 0248_ $$2sideral$$a107666 000074953 037__ $$aART-2018-107666 000074953 041__ $$aeng 000074953 100__ $$aSanz-Rubio, D. 000074953 245__ $$aStability of Circulating Exosomal miRNAs in Healthy Subjects article 000074953 260__ $$c2018 000074953 5060_ $$aAccess copy available to the general public$$fUnrestricted 000074953 5203_ $$aExosomes are nano-vesicles present in the circulation that are involved in cell-To-cell communication and regulation of different biological processes. MicroRNAs (miRNAs) are part of their cargo and are potential biomarkers. Methods of exosome isolation and the inter-individual and intra-individual variations in circulating miRNA exosomal cargo have been poorly investigated. This study aims for comparing two exosome isolation methods and to assess the stability of eleven plasma exosomal miRNAs over time. In addition to evaluate miRNA variability of both kits, the effect of freezing plasma before exosome isolation or freezing isolated exosomes on miRNA stability was also evaluated. MiRNA levels were tested in 7 healthy subjects who underwent four different blood extractions obtained in 4 consecutive weeks. One of the isolation kits displayed generally better amplification signals, and miRNAs from exosomes isolated after freezing the plasma had the highest levels. Intra-subject and inter-subject coefficients of variance were lower for the same isolation kit after freezing plasma. Finally, miRNAs that showed an acceptable expression level were stable across the consecutive extractions. This study shows for the first time the stability over time of miRNAs isolated from circulating plasma exosomes, establishing a key step in the use of exosomal miRNAs as biomarkers. 000074953 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-01940 000074953 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000074953 590__ $$a4.011$$b2018 000074953 591__ $$aMULTIDISCIPLINARY SCIENCES$$b14 / 69 = 0.203$$c2018$$dQ1$$eT1 000074953 592__ $$a1.414$$b2018 000074953 593__ $$aMultidisciplinary$$c2018$$dQ1 000074953 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000074953 700__ $$0(orcid)0000-0001-6016-4726$$aMartin-Burriel, I.$$uUniversidad de Zaragoza 000074953 700__ $$aGil, A. 000074953 700__ $$aCubero, P. 000074953 700__ $$aForner, M. 000074953 700__ $$aKhalyfa, A. 000074953 700__ $$0(orcid)0000-0001-9096-2294$$aMarin, J.M.$$uUniversidad de Zaragoza 000074953 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000074953 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina 000074953 773__ $$g8, 1 (2018), 10306 [10 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322 000074953 8564_ $$s1783713$$uhttps://zaguan.unizar.es/record/74953/files/texto_completo.pdf$$yVersión publicada 000074953 8564_ $$s115828$$uhttps://zaguan.unizar.es/record/74953/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000074953 909CO $$ooai:zaguan.unizar.es:74953$$particulos$$pdriver 000074953 951__ $$a2020-08-13-07:58:15 000074953 980__ $$aARTICLE