000074980 001__ 74980
000074980 005__ 20200117221636.0
000074980 0247_ $$2doi$$a10.3389/fcimb.2018.00236
000074980 0248_ $$2sideral$$a107508
000074980 037__ $$aART-2018-107508
000074980 041__ $$aeng
000074980 100__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña, A.$$uUniversidad de Zaragoza
000074980 245__ $$aHigh Throughput Sequencing and network analysis disentangle the microbial communities of ticks and hosts within and between ecosystems
000074980 260__ $$c2018
000074980 5060_ $$aAccess copy available to the general public$$fUnrestricted
000074980 5203_ $$aWe aimed to develop a framework, based on graph theory, to capture the ecological meaning behind pure pair comparisons of microbiome-derived data. As a proof of concept, we applied the framework to analyze the co-occurrence of bacteria in either Ixodes ricinus ticks or the spleen of one of their main hosts, the vole Myodes glareolus. As a secondary lymphoid organ, the spleen acts as a filter of blood and represents well the exposure to microorganisms circulating in the blood; including those acquired and transmitted by ticks during feeding. The microbiome of 301 and 269 individual tick and vole samples, respectively, were analyzed using next generation sequencing (NGS) of 16S rRNA. To assess the effect of habitat on ecological communities of bacteria associated to ticks and voles, two different biotopes were included in the study, forest, and ecotone. An innovative approach of NGS data analysis combining network analysis and phylogenies of co-occuring of bacteria was used to study associations between bacteria in individual samples. Of the 126 bacterial genera found in ticks and voles, 62% were shared by both species. Communities of co-occurring bacteria were always more phylogenetically diverse in ticks than in voles. Interestingly, ~80% of bacterial phylogenetic diversity was found in ~20% of ticks. This pattern was not observed in vole-associated bacteria. Results revealed that the microbiome of I. ricinus is only slightly related to that of M. glareolus and that the biotope plays the most important role in shaping the bacterial communities of either ticks or voles. The analysis of the phylogenetic signal of the network indexes across the 16S rRNA-derived tree of bacteria suggests that the microbiome of both ticks and voles has high phylogenetic diversity and that closest bacterial genera do not co-occur. This study shows that network analysis is a promising tool to unravel complex microbial communities associated to arthropod vectors and vertebrate hosts.
000074980 536__ $$9info:eu-repo/grantAgreement/EUR/COST/TD1303
000074980 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000074980 590__ $$a3.518$$b2018
000074980 591__ $$aMICROBIOLOGY$$b44 / 133 = 0.331$$c2018$$dQ2$$eT2
000074980 591__ $$aIMMUNOLOGY$$b69 / 157 = 0.439$$c2018$$dQ2$$eT2
000074980 592__ $$a1.541$$b2018
000074980 593__ $$aImmunology$$c2018$$dQ1
000074980 593__ $$aInfectious Diseases$$c2018$$dQ1
000074980 593__ $$aMicrobiology (medical)$$c2018$$dQ1
000074980 593__ $$aMicrobiology$$c2018$$dQ1
000074980 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000074980 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000074980 700__ $$aCabezas-Cruz, A.
000074980 700__ $$aPollet, T.
000074980 700__ $$aVayssier-Taussat, M.
000074980 700__ $$aCosson, J.F.
000074980 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000074980 773__ $$g8, JUL (2018), 236 [12 pp]$$tFrontiers in cellular and infection microbiology$$x2235-2988
000074980 8564_ $$s361589$$uhttps://zaguan.unizar.es/record/74980/files/texto_completo.pdf$$yVersión publicada
000074980 8564_ $$s12097$$uhttps://zaguan.unizar.es/record/74980/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000074980 909CO $$ooai:zaguan.unizar.es:74980$$particulos$$pdriver
000074980 951__ $$a2020-01-17-22:02:28
000074980 980__ $$aARTICLE