000075054 001__ 75054
000075054 005__ 20201117095023.0
000075054 0247_ $$2doi$$a10.5424/sjar/2018162-12882
000075054 0248_ $$2sideral$$a107590
000075054 037__ $$aART-2018-107590
000075054 041__ $$aeng
000075054 100__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, M.$$uUniversidad de Zaragoza
000075054 245__ $$aSperm quality and seminal plasma proteins in three sheep breeds under high altitude and tropical conditions
000075054 260__ $$c2018
000075054 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075054 5203_ $$aWe tested the hypothesis that sheep breed can influence the sperm quality and seminal plasma (SP) composition and investigated any potential relationship between SP proteins and antioxidant enzyme activities (AO) with sperm quality. Ejaculates from twelve rams of three breeds were obtained during the rainy season at high altitudes, and sperm quality was automatically evaluated (CASA-Hamilton Thorne). The AO of superoxide dismutase, glutathione peroxide and glutathione reductase (GR) in SP was evaluated and total proteins were separated by SDS-PAGE. Comparative analyses of semen quality parameters between breeds revealed that Creole and Hampshire breeds had a higher sperm quality compared with Romney Marsh (p<0.05), although no difference in AO was found. GR activity was negatively correlated (p<0.05) with several kinematic variables and positively (p<0.05) with morphological abnormalities. The highest SP protein concentration was found in semen collected from Hampshire males compared with that from Creole and Romney Marsh (p<0.05). SDS-PAGE analysis showed the presence of 32 protein bands in SP with molecular weights between 334 and 10 kDa. Differences (p<0.05) between breeds in bands of 43, 25, 22 and 20 kDa were observed. These results evidence a relationship between the protective effect of the antioxidant enzyme system in SP of three ram types under high altitude and tropical conditions and semen quality. Our findings also suggest that the identified proteins might play an important role in sperm physiology and quality.
000075054 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P
000075054 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000075054 590__ $$a1.035$$b2018
000075054 591__ $$aAGRICULTURE, MULTIDISCIPLINARY$$b28 / 56 = 0.5$$c2018$$dQ2$$eT2
000075054 592__ $$a0.391$$b2018
000075054 593__ $$aAgronomy and Crop Science$$c2018$$dQ2
000075054 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000075054 700__ $$aCardozo, J.A.
000075054 700__ $$aGrajales-Lombana, H.
000075054 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.A.$$uUniversidad de Zaragoza
000075054 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000075054 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000075054 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000075054 773__ $$g16, 2 (2018), e0403[13p]$$pSpan. j. agric. res.$$tSpanish Journal of Agricultural Research$$x1695-971X
000075054 8564_ $$s232561$$uhttps://zaguan.unizar.es/record/75054/files/texto_completo.pdf$$yVersión publicada
000075054 8564_ $$s117661$$uhttps://zaguan.unizar.es/record/75054/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000075054 909CO $$ooai:zaguan.unizar.es:75054$$particulos$$pdriver
000075054 951__ $$a2020-11-17-09:44:11
000075054 980__ $$aARTICLE