000075367 001__ 75367
000075367 005__ 20191105115759.0
000075367 0247_ $$2doi$$a10.1007/s11120-016-0327-x
000075367 0248_ $$2sideral$$a97836
000075367 037__ $$aART-2017-97836
000075367 041__ $$aeng
000075367 100__ $$aBernal-Bayard, P.
000075367 245__ $$aThe photosynthetic cytochrome c550 from the diatom Phaeodactylum tricornutum
000075367 260__ $$c2017
000075367 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075367 5203_ $$aThe photosynthetic cytochrome c550 from the marine diatom Phaeodactylum tricornutum has been purified and characterized. Cytochrome c550 is mostly obtained from the soluble cell extract in relatively large amounts. In addition, the protein appeared to be truncated in the last hydrophobic residues of the C-terminus, both in the soluble cytochrome c550 and in the protein extracted from the membrane fraction, as deduced by mass spectrometry analysis and the comparison with the gene sequence. Interestingly, it has been described that the C-terminus of cytochrome c550 forms a hydrophobic finger involved in the interaction with photosystem II in cyanobacteria. Cytochrome c550 was almost absent in solubilized photosystem II complex samples, in contrast with the PsbO and Psb31 extrinsic subunits, thus suggesting a lower affinity of cytochrome c550 for the photosystem II complex. Under iron-limiting conditions the amount of cytochrome c550 decreases up to about 45% as compared to iron-replete cells, pointing to an iron-regulated synthesis. Oxidized cytochrome c550 has been characterized using continuous wave EPR and pulse techniques, including HYSCORE, and the obtained results have been interpreted in terms of the electrostatic charge distribution in the surroundings of the heme centre.
000075367 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B18$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2012-35271$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2015-64169-P$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64486-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2011-23861
000075367 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000075367 590__ $$a3.091$$b2017
000075367 591__ $$aPLANT SCIENCES$$b39 / 222 = 0.176$$c2017$$dQ1$$eT1
000075367 592__ $$a1.359$$b2017
000075367 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000075367 593__ $$aPlant Science$$c2017$$dQ1
000075367 593__ $$aBiochemistry$$c2017$$dQ2
000075367 593__ $$aCell Biology$$c2017$$dQ2
000075367 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000075367 700__ $$aPuerto-Galán, L.
000075367 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, I.$$uUniversidad de Zaragoza
000075367 700__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, I.
000075367 700__ $$aCastell, C.
000075367 700__ $$aOrtega, J. M.
000075367 700__ $$0(orcid)0000-0003-3449-4929$$aAlonso, P. J.$$uUniversidad de Zaragoza
000075367 700__ $$aRoncel, M.
000075367 700__ $$0(orcid)0000-0002-5406-3280$$aMartínez, J. I.
000075367 700__ $$aHervás, M.
000075367 700__ $$aNavarro, J. A.
000075367 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000075367 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000075367 773__ $$g133, 1-3 (2017), 273-287$$pPhotosynth. res.$$tPHOTOSYNTHESIS RESEARCH$$x0166-8595
000075367 8564_ $$s4362926$$uhttps://zaguan.unizar.es/record/75367/files/texto_completo.pdf$$yPostprint
000075367 8564_ $$s56645$$uhttps://zaguan.unizar.es/record/75367/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000075367 909CO $$ooai:zaguan.unizar.es:75367$$particulos$$pdriver
000075367 951__ $$a2019-11-05-11:49:58
000075367 980__ $$aARTICLE