75367 20191105115759.0 doi 10.1007/s11120-016-0327-x sideral 97836 ART-2017-97836 eng Bernal-Bayard, P. The photosynthetic cytochrome c550 from the diatom Phaeodactylum tricornutum 2017 Access copy available to the general public Unrestricted The photosynthetic cytochrome c550 from the marine diatom Phaeodactylum tricornutum has been purified and characterized. Cytochrome c550 is mostly obtained from the soluble cell extract in relatively large amounts. In addition, the protein appeared to be truncated in the last hydrophobic residues of the C-terminus, both in the soluble cytochrome c550 and in the protein extracted from the membrane fraction, as deduced by mass spectrometry analysis and the comparison with the gene sequence. Interestingly, it has been described that the C-terminus of cytochrome c550 forms a hydrophobic finger involved in the interaction with photosystem II in cyanobacteria. Cytochrome c550 was almost absent in solubilized photosystem II complex samples, in contrast with the PsbO and Psb31 extrinsic subunits, thus suggesting a lower affinity of cytochrome c550 for the photosystem II complex. Under iron-limiting conditions the amount of cytochrome c550 decreases up to about 45% as compared to iron-replete cells, pointing to an iron-regulated synthesis. Oxidized cytochrome c550 has been characterized using continuous wave EPR and pulse techniques, including HYSCORE, and the obtained results have been interpreted in terms of the electrostatic charge distribution in the surroundings of the heme centre. info:eu-repo/grantAgreement/ES/DGA/B18 info:eu-repo/grantAgreement/ES/MINECO/BIO2012-35271 info:eu-repo/grantAgreement/ES/MINECO/BIO2015-64169-P info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64486-R info:eu-repo/grantAgreement/ES/MINECO/MAT2011-23861 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.091 2017 PLANT SCIENCES 39 / 222 = 0.176 2017 Q1 T1 1.359 2017 Medicine (miscellaneous) 2017 Q1 Plant Science 2017 Q1 Biochemistry 2017 Q2 Cell Biology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Puerto-Galán, L. (orcid)0000-0003-3608-4720 Yruela, I. Universidad de Zaragoza (orcid)0000-0002-1827-1250 García-Rubio, I. Castell, C. Ortega, J. M. (orcid)0000-0003-3449-4929 Alonso, P. J. Universidad de Zaragoza Roncel, M. (orcid)0000-0002-5406-3280 Martínez, J. I. Hervás, M. Navarro, J. A. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 133, 1-3 (2017), 273-287 Photosynth. res. PHOTOSYNTHESIS RESEARCH 0166-8595 4362926 http://zaguan.unizar.es/record/75367/files/texto_completo.pdf Postprint 56645 http://zaguan.unizar.es/record/75367/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:75367 articulos driver 2019-11-05-11:49:58 ARTICLE