75423 20200117212656.0 doi 10.1016/j.tiv.2017.10.016 sideral 101777 ART-2018-101777 eng (orcid)0000-0002-6134-8698 Sierra Arregui, Marta Oxidative stress prediction: A preliminary approach using a response surface based technique 2018 Access copy available to the general public Unrestricted A response surface was built to predict the lipid peroxidation level, generated in an iron-ascorbate in vitro model, of any organ, which is correlated with the oxidative stress injury in biological membranes. Oxidative stress studies are numerous, usually performed on laboratory animals. However, ethical concerns require validated methods to reduce the use of laboratory animals. The response surface described here is a validated method to replace animals. Tissue samples of rabbit liver, kidney, heart, skeletal muscle and brain were oxidized with different concentrations of FeCl3 (0.1 to 8 mM) and ascorbate (0.1 mM), during different periods of time (0 to 90 min) at 37 °C. Experimental data obtained, with lipid content and antioxidant activity of each organ, allowed constructing a multidimensional surface capable of predicting, by interpolation, the lipid peroxidation level of any organ defined by its antioxidant activity and fat content, when exposed to different oxidant conditions. To check the predictive potential of the technique, two more experiments were carried out. First, in vitro oxidation data from lung tissue were collected. Second, the antioxidant capacity of kidney homogenates was modified by adding melatonin. Then, the response surface generated could predict lipid peroxidation levels produced in these new situations. The potential of this technique could be reinforced using collaborative databases to reduce the number of animals in experimental procedures. info:eu-repo/grantAgreement/ES/UZ/JIUZ-2014-BIO-05 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.067 2018 TOXICOLOGY 36 / 93 = 0.387 2018 Q2 T2 0.895 2018 Toxicology 2018 Q1 Medicine (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Bragg-Gonzalo, Lorena (orcid)0000-0002-6870-0594 Grasa Orús, Jorge Universidad de Zaragoza (orcid)0000-0001-8301-6902 Muñoz Gonzalvo, María Jesús Universidad de Zaragoza (orcid)0000-0003-3003-5856 González Ibáñez, David Universidad de Zaragoza (orcid)0000-0001-5981-5448 Miana-Mena, Francisco Javier Universidad de Zaragoza 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1005 315 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Farmacología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 46 (2018), 273-283 Toxicol. in vitro TOXICOLOGY IN VITRO 0887-2333 890875 http://zaguan.unizar.es/record/75423/files/texto_completo.pdf Postprint 44515 http://zaguan.unizar.es/record/75423/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:75423 articulos driver 2020-01-17-21:23:05 ARTICLE