000075449 001__ 75449
000075449 005__ 20200324135216.0
000075449 0247_ $$2doi$$a10.3389/fmats.2015.00037
000075449 0248_ $$2sideral$$a107011
000075449 037__ $$aART-2015-107011
000075449 041__ $$aeng
000075449 100__ $$0(orcid)0000-0002-9414-1845$$aAyuso, Jose Maria$$uUniversidad de Zaragoza
000075449 245__ $$aSU-8 Based Microdevices to Study Self-Induced Chemotaxis in 3D Microenvironments
000075449 260__ $$c2015
000075449 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075449 5203_ $$aTissues are complex three-dimensional structures in which cell behavior is frequently guided by chemotactic signals. Although starvation and nutrient restriction induce many different chemotactic processes, the recreation of such conditions in vitro remains difficult when using standard cell culture equipment. Recently, microfluidic techniques have arisen as powerful tools to mimic such physiological conditions. In this context, microfluidic three-dimensional cell culture systems require precise control of cell/hydrogel location because samples need to be placed within a microchamber without obstruction of surrounding elements. In this article, SU-8 is studied as structural material for the fabrication of complex cell culture microdevices due to its good mechanical properties and sensor integration capacity. Moreover, SU-8 physical properties and their effect on a successful design for precise control of hydrogel location within microfluidic devices are studied. In particular, this manuscript presents a SU-8 based microdevice designed to create “self-induced” medium starvation, based on the combination of nutrient restriction and natural cell metabolism. Results show a natural migratory response toward nutrient source, showing how cells adapt to their own microenvironment modifications. The presented results demonstrate the SU-8 potential for microdevice fabrication applied to cell culture.
000075449 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BES-2012-059940$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2011-28262-C04-01
000075449 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000075449 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000075449 700__ $$aMonge, Rosa
000075449 700__ $$0(orcid)0000-0002-5731-8840$$aLlamazares, Guillermo A.$$uUniversidad de Zaragoza
000075449 700__ $$aMoreno, Marco
000075449 700__ $$aAgirregabiria, Maria
000075449 700__ $$aBerganzo, Javier
000075449 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000075449 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000075449 700__ $$0(orcid)0000-0001-5376-4440$$aFernández, Luis J.$$uUniversidad de Zaragoza
000075449 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000075449 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000075449 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000075449 773__ $$g2 (2015), 37$$pFront. mater.$$tFrontiers in Materials$$x2296-8016
000075449 8564_ $$s12181396$$uhttps://zaguan.unizar.es/record/75449/files/texto_completo.pdf$$yVersión publicada
000075449 8564_ $$s9196$$uhttps://zaguan.unizar.es/record/75449/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000075449 909CO $$ooai:zaguan.unizar.es:75449$$particulos$$pdriver
000075449 951__ $$a2020-03-24-10:01:43
000075449 980__ $$aARTICLE