75449 20200324135216.0 doi 10.3389/fmats.2015.00037 sideral 107011 ART-2015-107011 eng (orcid)0000-0002-9414-1845 Ayuso, Jose Maria Universidad de Zaragoza SU-8 Based Microdevices to Study Self-Induced Chemotaxis in 3D Microenvironments 2015 Access copy available to the general public Unrestricted Tissues are complex three-dimensional structures in which cell behavior is frequently guided by chemotactic signals. Although starvation and nutrient restriction induce many different chemotactic processes, the recreation of such conditions in vitro remains difficult when using standard cell culture equipment. Recently, microfluidic techniques have arisen as powerful tools to mimic such physiological conditions. In this context, microfluidic three-dimensional cell culture systems require precise control of cell/hydrogel location because samples need to be placed within a microchamber without obstruction of surrounding elements. In this article, SU-8 is studied as structural material for the fabrication of complex cell culture microdevices due to its good mechanical properties and sensor integration capacity. Moreover, SU-8 physical properties and their effect on a successful design for precise control of hydrogel location within microfluidic devices are studied. In particular, this manuscript presents a SU-8 based microdevice designed to create “self-induced” medium starvation, based on the combination of nutrient restriction and natural cell metabolism. Results show a natural migratory response toward nutrient source, showing how cells adapt to their own microenvironment modifications. The presented results demonstrate the SU-8 potential for microdevice fabrication applied to cell culture. info:eu-repo/grantAgreement/ES/MINECO/BES-2012-059940 info:eu-repo/grantAgreement/ES/MINECO/DPI2011-28262-C04-01 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Monge, Rosa (orcid)0000-0002-5731-8840 Llamazares, Guillermo A. Universidad de Zaragoza Moreno, Marco Agirregabiria, Maria Berganzo, Javier (orcid)0000-0001-8741-6452 Doblaré, Manuel Universidad de Zaragoza (orcid)0000-0003-2410-5678 Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0001-5376-4440 Fernández, Luis J. Universidad de Zaragoza 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 2 (2015), 37 Front. mater. Frontiers in Materials 2296-8016 12181396 http://zaguan.unizar.es/record/75449/files/texto_completo.pdf Versión publicada 9196 http://zaguan.unizar.es/record/75449/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:75449 articulos driver 2020-03-24-10:01:43 ARTICLE