000075476 001__ 75476
000075476 005__ 20200616135721.0
000075476 0247_ $$2doi$$a10.1002/bit.26784
000075476 0248_ $$2sideral$$a108185
000075476 037__ $$aART-2018-108185
000075476 041__ $$aeng
000075476 100__ $$aChen, C.
000075476 245__ $$aHepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers
000075476 260__ $$c2018
000075476 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075476 5203_ $$aDirect reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes. The resulting iHeps are homogenous and display key properties of primary hepatocytes, such as expression of hepatocyte markers, albumin secretion, and presence of liver transaminases. iHeps also possess the capacity to repopulate decellularized liver tissue and exhibit enhanced hepatic maturation. As such, we present a novel strategy to generate homogenous and functional iHeps for applications in tissue engineering and cell therapy.
000075476 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-00563$$9info:eu-repo/grantAgreement/ES/ISCIII/EH16PI02$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 660554-Liver Bioengineering$$9info:eu-repo/grantAgreement/EC/H2020/660554/EU/Ex vivo Re-vascularization in Porcine Liver Bioengineering - A Critical First Step Towards Effective Transplantation of Bioengineered Livers/Liver Bioengineering
000075476 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000075476 590__ $$a4.26$$b2018
000075476 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b30 / 162 = 0.185$$c2018$$dQ1$$eT1
000075476 592__ $$a1.309$$b2018
000075476 593__ $$aApplied Microbiology and Biotechnology$$c2018$$dQ1
000075476 593__ $$aBiotechnology$$c2018$$dQ1
000075476 593__ $$aBioengineering$$c2018$$dQ1
000075476 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000075476 700__ $$aPla-Palacín, I.
000075476 700__ $$0(orcid)0000-0002-8969-7726$$aBaptista, P.M.
000075476 700__ $$aShang, P.
000075476 700__ $$aOosterhoff, L.A.
000075476 700__ $$avan Wolferen, M.E.
000075476 700__ $$aPenning, L.C.
000075476 700__ $$aGeijsen, N.
000075476 700__ $$aSpee, B.
000075476 773__ $$g115, 11 (2018), 2807 - 2816$$pBiotechnol. bioeng.$$tBIOTECHNOLOGY AND BIOENGINEERING$$x0006-3592
000075476 8564_ $$s949349$$uhttps://zaguan.unizar.es/record/75476/files/texto_completo.pdf$$yVersión publicada
000075476 8564_ $$s90007$$uhttps://zaguan.unizar.es/record/75476/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000075476 909CO $$ooai:zaguan.unizar.es:75476$$particulos$$pdriver
000075476 951__ $$a2020-06-16-13:49:30
000075476 980__ $$aARTICLE