Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers

Chen, C. ; Pla-Palacín, I. ; Baptista, P.M. ; Shang, P. ; Oosterhoff, L.A. ; van Wolferen, M.E. ; Penning, L.C. ; Geijsen, N. ; Spee, B.

Resumen: Direct reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes. The resulting iHeps are homogenous and display key properties of primary hepatocytes, such as expression of hepatocyte markers, albumin secretion, and presence of liver transaminases. iHeps also possess the capacity to repopulate decellularized liver tissue and exhibit enhanced hepatic maturation. As such, we present a novel strategy to generate homogenous and functional iHeps for applications in tissue engineering and cell therapy.

Idioma: Inglés

DOI: 10.1002/bit.26784

Año: 2018

Publicado en: BIOTECHNOLOGY AND BIOENGINEERING 115, 11 (2018), 2807 - 2816
ISSN: 0006-3592

Factor impacto JCR: 4.26 (2018)

Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 30 / 162 = 0.185 (2018) - Q1 - T1

Factor impacto SCIMAGO: 1.309 - Applied Microbiology and Biotechnology (Q1) - Biotechnology (Q1) - Bioengineering (Q1)

Financiación: info:eu-repo/grantAgreement/EC/H2020/660554/EU/Ex vivo Re-vascularization in Porcine Liver Bioengineering - A Critical First Step Towards Effective Transplantation of Bioengineered Livers/Liver Bioengineering
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/EH16PI02
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-00563

Tipo y forma: Article (Published version)