000075618 001__ 75618
000075618 005__ 20190709135542.0
000075618 0247_ $$2doi$$a10.1093/aob/mcw239
000075618 0248_ $$2sideral$$a105139
000075618 037__ $$aART-2017-105139
000075618 041__ $$aeng
000075618 100__ $$0(orcid)0000-0002-7734-8481$$aLopez-Alvarez, D.
000075618 245__ $$aDiversity and association of phenotypic and metabolomic traits in the close model grasses Brachypodium distachyon, B. stacei and B. hybridum
000075618 260__ $$c2017
000075618 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075618 5203_ $$aBackground and Aims 
Morphological traits in combination with metabolite fingerprinting were used to investigate inter-and intraspecies diversity within the model annual grasses Brachypodium distachyon, Brachypodium stacei and Brachypodium hybridum. 
Phenotypic variation of 15 morphological characters and 2219 nominal mass (m/z) signals generated using flow infusion electrospray ionization-mass spectrometry (FIE-MS) were evaluated in individuals from a total of 174 wild populations and six inbred lines, and 12 lines, of the three species, respectively. Basic statistics and multivariate principal component analysis and discriminant analysis were used to differentiate inter-and intraspecific variability of the two types of variable, and their association was assayed with the rcorr function. Key 
Basic statistics and analysis of variance detected eight phenotypic characters [(stomata) leaf guard cell length, pollen grain length, (plant) height, second leaf width, inflorescence length, number of spikelets per inflorescence, lemma length, awn length] and 434 tentatively annotated metabolite signals that significantly discriminated the three species. Three phenotypic traits (pollen grain length, spikelet length, number of flowers per inflorescence) might be genetically fixed. The three species showed different metabolomic profiles. Discriminant analysis significantly discriminated the three taxa with both morphometric and metabolome traits and the intraspecific phenotypic diversity within B. distachyon and B. stacei. The populations of B. hybridum were considerably less differentiated. 
Highly explanatory metabolite signals together with morphological characters revealed concordant patterns of differentiation of the three taxa. Intraspecific phenotypic diversity was observed between northern and southern Iberian populations of B. distachyon and between eastern Mediterranean/south-western Asian and western Mediterranean populations of B. stacei. Significant association was found for pollen grain length and lemma length and ten and six metabolomic signals, respectively. These results would guide the selection of new germplasm lines of the three model grasses in ongoing genome-wide association studies.
000075618 536__ $$9info:eu-repo/grantAgreement/ES/DGA/Bioflora$$9info:eu-repo/grantAgreement/ES/MINECO/CGL2009-12955-C02-01$$9info:eu-repo/grantAgreement/ES/MINECO/CGL2012-39953-C02-01
000075618 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000075618 590__ $$a3.646$$b2017
000075618 591__ $$aPLANT SCIENCES$$b27 / 222 = 0.122$$c2017$$dQ1$$eT1
000075618 592__ $$a1.721$$b2017
000075618 593__ $$aPlant Science$$c2017$$dQ1
000075618 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000075618 700__ $$aZubair, H.
000075618 700__ $$aBeckmann, M.
000075618 700__ $$aDraper, J.
000075618 700__ $$0(orcid)0000-0001-7793-5259$$aCatalan, P.$$uUniversidad de Zaragoza
000075618 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000075618 773__ $$g119, 4 (2017), 545-561$$pAnn. bot.$$tANNALS OF BOTANY$$x0305-7364
000075618 8564_ $$s848959$$uhttps://zaguan.unizar.es/record/75618/files/texto_completo.pdf$$yPostprint
000075618 8564_ $$s126374$$uhttps://zaguan.unizar.es/record/75618/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000075618 909CO $$ooai:zaguan.unizar.es:75618$$particulos$$pdriver
000075618 951__ $$a2019-07-09-12:08:06
000075618 980__ $$aARTICLE