Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources

Alonso, C.A. ; Michael, G.B. ; Li, J. ; Somalo, S. ; Simón, C. (Universidad de Zaragoza) ; Wang, Y. ; Kaspar, H. ; Kadlec, K. ; Torres, C. ; Schwarz, S.

Resumen: Objectives: This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective blaSHV-12-carrying plasmids and sequencing one of these plasmids completely.
Methods: Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed blaSHV-12-carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the blaSHV-12 genetic environment were analysed by PCR and sequencing. One IncI1 plasmid was sequenced completely using HiSeq 2500 and gap closure by PCRs and Sanger sequencing.
Results: Among the 23 SHV-12-positive E. coli, some isolates from different sources showed the same characteristics: ST23/phylogroup A (human, dog, livestock), ST57/D (wild bird, chicken meat) and ST117/D (chicken meat, chicken). All blaSHV-12 genes were horizontally transferable via 30-120 kb plasmids of incompatibility groups IncI1 (n=17), IncK (n=3), IncF (n=1), IncX3 (n=1) and a non-typeable plasmid. IncK plasmids, indistinguishable in size and restriction patterns, were found in isolates from different sources (ST57/D, meat; ST131/B2, meat; ST57/B1, dog). The IncI1-blaSHV-12-carrying plasmids were mostly assigned to plasmid ST (pST) 26 and pST3. Three plasmids showed novel pSTs (pST214, pST215). The majority of the IncI1 transformants exhibited resistance to ß-lactams, chloramphenicol and streptomycin (in relation with a class 1 integron containing an estX-psp-aadA2-cmlA1-aadA1-qacI gene cassette array), and to tetracycline. A novel blaSHV-12 environment was detected and whole plasmid sequencing revealed a Tn21-derived-blaSHV12-¿Tn1721 resistance complex.
Conclusions: Results from this study suggest that the dissemination of blaSHV-12 genes occurs by vertical (clonal) and horizontal transfer, the latter mainly mediated through IncI1 multidrug-resistance plasmids.

Idioma: Inglés

DOI: 10.1093/jac/dkx024

Año: 2017

Publicado en: The Journal of antimicrobial chemotherapy 72, 6 (2017), 1589-1596
ISSN: 0305-7453

Factor impacto JCR: 5.217 (2017)

Categ. JCR: INFECTIOUS DISEASES rank: 8 / 88 = 0.091 (2017) - Q1 - T1
Categ. JCR: PHARMACOLOGY & PHARMACY rank: 19 / 261 = 0.073 (2017) - Q1 - T1
Categ. JCR: MICROBIOLOGY rank: 19 / 125 = 0.152 (2017) - Q1 - T1

Factor impacto SCIMAGO: 2.419 - Infectious Diseases (Q1) - Pharmacology (medical) (Q1) - Pharmacology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/BES-2013–063105
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76571-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)