000075642 001__ 75642
000075642 005__ 20200221144340.0
000075642 0247_ $$2doi$$a10.1016/j.toxlet.2016.06.1965
000075642 0248_ $$2sideral$$a106202
000075642 037__ $$aART-2016-106202
000075642 041__ $$aeng
000075642 100__ $$aAimonen, K.
000075642 245__ $$aIn vivo genotoxicity and inflammatory effects of uncoated and coated CeO2 NPs in mice
000075642 260__ $$c2016
000075642 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075642 5203_ $$aP17-045
Ceria nanoparticles (CeO2 NPs) have several industrial applications and pharmacological potential due to their antioxidant properties. However, toxicity data on CeO2 NPs are scarce and show contradictory results. In the present study, uncoated, polyethylene glycol- and citrate-coated CeO2 NPs (4-8 nm) were administrated to C57Bl/6 mice by repeated dose (3×) pharyngeal aspiration using four different doses of each type of NPs (corresponding to 4.4, 8.8, 17.6 and 35.2 µg Ce2+/mouse/aspiration), and sampled 1 and 28 days after the last administration. DNA damage was assessed by the comet assay locally in bronchoalveolar lavage (BAL) and lung cells, and systemically in liver cells. Micronuclei, a biomarker of chromosome damage, were analysed in bone marrow and peripheral blood erythrocytes. Immunotoxicity was evaluated by BAL cell counting. Furthermore, histopathological effects on the lungs and biodistribution of the NPs (analysis of Ce2+ in several organs) were assessed. At 24-h, a significant increase in DNA damage was induced at the highest doses by uncoated and citrate-coated NPs in BAL cells. For these NPs a significant, but non-dose-dependent, effect was observed in lung and liver cells at 28-d. No systemic genotoxic effects were observed with any of the NPs. A dose-dependent accumulation of macrophages and activated lymphocytes was seen in the lungs for all the NPs, although a milder reaction was elicited by the coated NPs. Our findings show that short-term exposure of mice to CeO2 NPs induces pulmonary inflammation, and non-dose-dependent DNA damage, but no systemic genotoxicity. (Funded by the EU FP-7 GUIDEnano, Grant Agreement No.604387).
000075642 536__ $$9info:eu-repo/grantAgreement/EC/FP7/604387/EU/Assessment and mitigation of nano-enabled product risks on human and environmental health: Development of new strategies and creation of a digital guidance tool for nanotech industries/GUIDENANO
000075642 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000075642 590__ $$a3.858$$b2016
000075642 591__ $$aTOXICOLOGY$$b14 / 92 = 0.152$$c2016$$dQ1$$eT1
000075642 592__ $$a1.302$$b2016
000075642 593__ $$aToxicology$$c2016$$dQ1
000075642 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000075642 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000075642 700__ $$aCatalan, J.
000075642 700__ $$aSuhonen, S.
000075642 700__ $$aHartikainen, M.
000075642 700__ $$aVippola, M.
000075642 700__ $$0(orcid)0000-0002-1946-1187$$aMoreno, C.$$uUniversidad de Zaragoza
000075642 700__ $$aCabellos, J.
000075642 700__ $$aJaner, G.
000075642 700__ $$aCampos, S.V.
000075642 700__ $$aWolff, H.
000075642 700__ $$aSavolainen, K.
000075642 700__ $$aNorppa, H.
000075642 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000075642 773__ $$g258, Suppl. (2016), S276 [P17-045]$$pToxicol. lett.$$tTOXICOLOGY LETTERS$$x0378-4274
000075642 8564_ $$s79615$$uhttps://zaguan.unizar.es/record/75642/files/texto_completo.pdf$$yVersión publicada
000075642 8564_ $$s132763$$uhttps://zaguan.unizar.es/record/75642/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000075642 909CO $$ooai:zaguan.unizar.es:75642$$particulos$$pdriver
000075642 951__ $$a2020-02-21-13:49:23
000075642 980__ $$aARTICLE