75705 20190709135701.0 doi 10.2217/nnm-2017-0198 sideral 103538 ART-2017-103538 eng Schwinté, P. Anti-inflammatory effect of active nanofibrous polymeric membrane bearing nanocontainers of atorvastatin complexes 2017 Access copy available to the general public Unrestricted Aim: We developed polymeric membranes for local administration of nonsoluble anti-inflammatory statin, as potential wound patch in rheumatic joint or periodontal lesions. Methods: Electrospun polycaprolactone membranes were fitted with polysaccharide-atorvastatin nanoreservoirs by using complexes with poly-aminocyclodextrin. Characterization methods are UV-Visible and X-ray photoelectron spectroscopy, molecular dynamics, scanning and transmission electron microscopy. In vitro, membranes were seeded with macrophages, and inflammatory cytokine expression were monitored. Results & conclusion: Stable inclusion complexes were formed in solution (1:1 stability constant 368 M- 1, -117.40 kJ mol- 1), with supramolecular globular organization (100 nm, substructure 30 nm). Nanoreservoir technology leads to homogeneous distribution of atorvastatin calcium trihydrate complexes in the membrane. Quantity embedded was estimated (70-90 µg in 30 µm × 6 mm membrane). Anti-inflammatory effect by cell contact-dependent release reached 60% inhibition for TNF-a and 80% for IL-6. The novelty resides in the double protection offered by the cyclodextrins as drug molecular chaperones, with further embedding into biodegradable nanoreservoirs. The strategy is versatile and can target other diseases. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.005 2017 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 22 / 160 = 0.138 2017 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 32 / 92 = 0.348 2017 Q2 T2 1.302 2017 Bioengineering 2017 Q1 Biomedical Engineering 2017 Q1 Development 2017 Q1 Materials Science (miscellaneous) 2017 Q1 Medicine (miscellaneous) 2017 Q1 Nanoscience and Nanotechnology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Mariotte, A. Anand, P. Keller, L. Idoux-Gillet, Y. Huck, O. Fioretti, F. Tenenbaum, H. Georgel, P. Wenzel, W. (orcid)0000-0002-2966-9088 Irusta, S. Universidad de Zaragoza Benkirane-Jessel, N. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 12, 23 (2017), 2651-2674 Nanomedicine Nanomedicine 1743-5889 2114744 http://zaguan.unizar.es/record/75705/files/texto_completo.pdf Postprint 35820 http://zaguan.unizar.es/record/75705/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:75705 articulos driver 2019-07-09-12:49:12 ARTICLE