Effect of cryopreserved amniotic membrane on the mechanical properties of skeletal muscle after strabismus surgery in rabbits

Sierra, M. (Universidad de Zaragoza) ; Ortillés, A. (Universidad de Zaragoza) ; Miana-Mena, F.J. (Universidad de Zaragoza) ; Grasa, J. (Universidad de Zaragoza) ; Calvo, B. (Universidad de Zaragoza)

Resumen: Purpose: To study the functional recovery of the superior rectus muscle (SRM) after its partial resection in a rabbit model with and without cryopreserved amniotic membrane (AM).
Material and methods: Resection of the right and left SRMs of 30 rabbits was performed. On the left eyes, a single sheet of equine cryopreserved AM was placed covering the muscle edge sutured. Active and passive mechanical properties of muscles operated with and without AM were monitored over time at 30 (n = 10), 60 (n = 10), and 90 (n = 10) days after surgery. Muscle samples were extracted and electrically stimulated to register the force exerted by the samples, characterizing its active behavior. They were, then, subjected to stretching test to obtain its resistance to deformation, known as passive behavior. Moreover, right and left eyes of a control group (n = 5) were equally subjected to active and passive tests to characterize the physiological behavior of SRM muscles.
Results: On active function examination, statistically significant differences were documented between the following: control vs AM and no AM at 30 days (p = 0.002 and p = 0.04, respectively). All other comparisons were insignificant (p > 0.05). On passive function analysis, significant differences were only found between control vs. no AM at 30 days (p = 0.004) and between AM vs. no AM at 30 days (p = 0.002). Indeed, muscle operated without AM did not recover a normal passive function until 60 days after surgery.
Conclusion: Cryopreserved AM is effective in accelerating recovery of SRM passive function in rabbits. Nevertheless, AM produced no significant effect on recovery of SRM active function.¿

Idioma: Inglés

DOI: 10.1080/02713683.2017.1387272

Año: 2017

Publicado en: CURRENT EYE RESEARCH 43, 2 (2017), 193-199
ISSN: 0271-3683

Factor impacto JCR: 2.12 (2017)

Categ. JCR: OPHTHALMOLOGY rank: 27 / 59 = 0.458 (2017) - Q2 - T2

Factor impacto SCIMAGO: 0.956 - Ophthalmology (Q2) - Sensory Systems (Q3) - Cellular and Molecular Neuroscience (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA/T88
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2017-84047-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Farmacología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)