000075740 001__ 75740
000075740 005__ 20190709135652.0
000075740 0247_ $$2doi$$a10.1021/acsami.7b14812
000075740 0248_ $$2sideral$$a104280
000075740 037__ $$aART-2017-104280
000075740 041__ $$aeng
000075740 100__ $$0(orcid)0000-0002-2576-6382$$aPrieto, Martín
000075740 245__ $$aLight emitting photon-upconversion nanoparticles in the generation of transdermal reactive oxygen species
000075740 260__ $$c2017
000075740 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075740 5203_ $$aCommon photosensitizers used in photodynamic therapy do not penetrate the skin effectively. In addition, the visible blue and red lights used to excite such photosensitizers have shallow penetration depths through tissue. To overcome these limitations, we have synthesized ultraviolet- and visible-light-emitting, energy-transfer-based upconversion nanoparticles and coencapsulated them inside PLGA-PEG (methoxy poly(ethylene glycol)-b-poly(lactic-co-glycolic acid)) nanoparticles with the photosensitizer protoporphyrin IX. Nd3+ has been introduced as a sensitizer in the upconversion nanostructure to allow its excitation at 808 nm. The subcytotoxic doses of the hybrid nanoparticles have been evaluated on different cell lines (i.e., fibroblasts, HaCaT, THP-1 monocytic cell line, U251MG (glioblastoma cell line), and mMSCs (murine mesenchymal stem cells). Upon NIR (near infrared)-light excitation, the upconversion nanoparticles emitted UV and VIS light, which consequently activated the generation of reactive-oxygen species (ROS). In addition, after irradiating at 808 nm, the resulting hybrid nanoparticles containing both upconversion nanoparticles and protoporphyrin IX generated 3.4 times more ROS than PLGA-PEG nanoparticles containing just the same dose of protoporphyrin IX. Their photodynamic effect was also assayed on different cell cultures, demonstrating their efficacy in selectively killing treated and irradiated cells. Compared to the topical application of the free photosensitizer, enhanced skin permeation and penetration were observed for the nanoparticulate formulation, using an ex vivo human-skin-permeation experiment. Whereas free protoporphyrin IX remained located at the outer layer of the skin, nanoparticle-encapsulated protoporphyrin IX was able to penetrate through the epidermal layer slightly into the dermis.
000075740 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
000075740 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000075740 590__ $$a8.097$$b2017
000075740 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b15 / 92 = 0.163$$c2017$$dQ1$$eT1
000075740 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b26 / 285 = 0.091$$c2017$$dQ1$$eT1
000075740 592__ $$a2.784$$b2017
000075740 593__ $$aMaterials Science (miscellaneous)$$c2017$$dQ1
000075740 593__ $$aNanoscience and Nanotechnology$$c2017$$dQ1
000075740 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000075740 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000075740 700__ $$aRwei, Alina Y.
000075740 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa
000075740 700__ $$aWei, Tuo
000075740 700__ $$aLópez-Franco, María Teresa
000075740 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000075740 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000075740 700__ $$aKohane, Daniel S.
000075740 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000075740 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000075740 773__ $$g9, 48 (2017), 41737-41747$$pACS appl. mater. interfaces$$tACS Applied Materials & Interfaces$$x1944-8244
000075740 8564_ $$s1054479$$uhttps://zaguan.unizar.es/record/75740/files/texto_completo.pdf$$yPostprint
000075740 8564_ $$s47941$$uhttps://zaguan.unizar.es/record/75740/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000075740 909CO $$ooai:zaguan.unizar.es:75740$$particulos$$pdriver
000075740 951__ $$a2019-07-09-12:44:05
000075740 980__ $$aARTICLE