000075802 001__ 75802
000075802 005__ 20191212102141.0
000075802 0247_ $$2doi$$a10.3389/fmicb.2018.02293
000075802 0248_ $$2sideral$$a108478
000075802 037__ $$aART-2018-108478
000075802 041__ $$aeng
000075802 100__ $$aSalvador-Bescos, M.
000075802 245__ $$aWadD, a New Brucella Lipopolysaccharide Core Glycosyltransferase Identified by Genomic Search and Phenotypic Characterization
000075802 260__ $$c2018
000075802 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075802 5203_ $$aBrucellosis, an infectious disease caused by Brucella, is one of the most extended bacterial zoonosis in the world and an important cause of economic losses and human suffering. The lipopolysaccharide (LPS) of Brucella plays a major role in virulence as it impairs normal recognition by the innate immune system and delays the immune response. The LPS core is a branched structure involved in resistance to complement and polycationic peptides, and mutants in glycosyltransferases required for the synthesis of the lateral branch not linked to the O-polysaccharide (O-PS) are attenuated and have been proposed as vaccine candidates. For this reason, the complete understanding of the genes involved in the synthesis of this LPS section is of particular interest. The chemical structure of the Brucella LPS core suggests that, in addition to the already identified WadB and WadC glycosyltransferases, others could be implicated in the synthesis of this lateral branch. To clarify this point, we identified and constructed mutants in 11 ORFs encoding putative glycosyltransferases in B. abortus. Four of these ORFs, regulated by the virulence regulator MucR (involved in LPS synthesis) or the ByrR/ByrS system (implicated in the synthesis of surface components), were not required for the synthesis of a complete LPS neither for virulence or interaction with polycationic peptides and/or complement. Among the other seven ORFs, six seemed not to be required for the synthesis of the core LPS since the corresponding mutants kept the O-PS and reacted as the wild type with polyclonal sera. Interestingly, mutant in ORF BAB1_0953 (renamed wadD) lost reactivity against antibodies that recognize the core section while kept the O-PS. This suggests that WadD is a new glycosyltransferase adding one or more sugars to the core lateral branch. WadD mutants were more sensitive than the parental strain to components of the innate immune system and played a role in chronic stages of infection. These results corroborate and extend previous work indicating that the Brucella LPS core is a branched structure that constitutes a steric impairment preventing the elements of the innate immune system to fight against Brucella.
000075802 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2008-04514-C03-03-GAN$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-3-R
000075802 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000075802 590__ $$a4.259$$b2018
000075802 591__ $$aMICROBIOLOGY$$b32 / 133 = 0.241$$c2018$$dQ1$$eT1
000075802 592__ $$a1.633$$b2018
000075802 593__ $$aMicrobiology (medical)$$c2018$$dQ1
000075802 593__ $$aMicrobiology$$c2018$$dQ1
000075802 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000075802 700__ $$aGil-Ramirez, Y.
000075802 700__ $$aZuniga-Ripa, A.
000075802 700__ $$aMartinez-Gomez, E.
000075802 700__ $$ade Miguel, M.J.
000075802 700__ $$0(orcid)0000-0002-7970-4929$$aMuñoz, P.M.
000075802 700__ $$aCloeckaert, A.
000075802 700__ $$aZygmunt, M.S.
000075802 700__ $$aMoriyon, I.
000075802 700__ $$aIriarte, M.
000075802 700__ $$aConde-Alvarez, R.
000075802 773__ $$g9 (2018), 2293 [14 pp]$$pFront. microbiol.$$tFRONTIERS IN MICROBIOLOGY$$x1664-302X
000075802 8564_ $$s339841$$uhttps://zaguan.unizar.es/record/75802/files/texto_completo.pdf$$yVersión publicada
000075802 8564_ $$s29795$$uhttps://zaguan.unizar.es/record/75802/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000075802 951__ $$a2019-12-12-10:12:39
000075802 980__ $$aARTICLE