75810 20191212102146.0 doi 10.1073/pnas.1804198115 sideral 108518 ART-2018-108518 eng Pujols, J. Small molecule inhibits alpha-synuclein aggregation, disrupts amyloid fibrils, and prevents degeneration of dopaminergic neurons 2018 Access copy available to the general public Unrestricted Parkinson''s disease (PD) is characterized by a progressive loss of dopaminergic neurons, a process that current therapeutic approaches cannot prevent. In PD, the typical pathological hallmark is the accumulation of intracellular protein inclusions, known as Lewy bodies and Lewy neurites, which are mainly composed of alpha-synuclein. Here, we exploited a high-throughput screening methodology to identify a small molecule (SynuClean-D) able to inhibit alpha-synuclein aggregation. SynuClean-D significantly reduces the in vitro aggregation of wildtype alpha-synuclein and the familiar A30P and H50Q variants in a substoichiometric molar ratio. This compound prevents fibril propagation in protein-misfolding cyclic amplification assays and decreases the number of alpha-synuclein inclusions in human neuroglioma cells. Computational analysis suggests that SynuClean-D can bind to cavities in mature alpha-synuclein fibrils and, indeed, it displays a strong fibril disaggregation activity. The treatment with SynuClean-D of two PD Caenorhabditis elegans models, expressing alpha-synuclein either in muscle or in dopaminergic neurons, significantly reduces the toxicity exerted by alpha- synuclein. SynuClean-D-treated worms show decreased alpha-synuclein aggregation in muscle and a concomitant motility recovery. More importantly, this compound is able to rescue dopaminergic neurons from alpha-synuclein-induced degeneration. Overall, SynuClean-D appears to be a promising molecule for therapeutic intervention in Parkinson's disease. info:eu-repo/grantAgreement/ES/DGA/E45-17R info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 9.58 2018 MULTIDISCIPLINARY SCIENCES 7 / 69 = 0.101 2018 Q1 T1 5.601 2018 Multidisciplinary 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pena-Diaz, S. Lazaro, D.F. Peccati, F. Pinheiro, F. Gonzalez, D. Carija, A. Navarro, S. (orcid)0000-0003-4358-9110 Conde-Gimenez, M. Garcia, J. Guardiola, S. Giralt, E. Salvatella, X. (orcid)0000-0002-2879-9200 Sancho, J. Universidad de Zaragoza Sodupe, M. Outeiro, T.F. Dalfo, E. Ventura, S. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 115, 41 (2018), 10481-10486 Proc. Natl. Acad. Sci. Proceedings of the National Academy of Sciences 0027-8424 818407 http://zaguan.unizar.es/record/75810/files/texto_completo.pdf Versión publicada 146308 http://zaguan.unizar.es/record/75810/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:75810 articulos driver 2019-12-12-10:15:08 ARTICLE