000075898 001__ 75898
000075898 005__ 20190709135653.0
000075898 0247_ $$2doi$$a10.1093/mutage/gew035
000075898 0248_ $$2sideral$$a105586
000075898 037__ $$aART-2017-105586
000075898 041__ $$aeng
000075898 100__ $$0(orcid)0000-0003-2936-242X$$aCatalan, J.$$uUniversidad de Zaragoza
000075898 245__ $$aGenotoxic and inflammatory effects of nanofibrillated cellulose in murine lungs
000075898 260__ $$c2017
000075898 5060_ $$aAccess copy available to the general public$$fUnrestricted
000075898 5203_ $$aNanofibrillated cellulose (NFC) is a sustainable and renewable nanomaterial, with diverse potential applications in the paper and medical industries. As NFC consists of long fibres of high aspect ratio, we examined here whether TEMPO-(2, 2, 6, 6-tetramethyl-piperidin-1-oxyl) oxidised NFC (length 300-1000 nm, thickness 10-25 nm), administrated by a single pharyngeal aspiration, could be genotoxic to mice, locally in the lungs or systemically in the bone marrow. Female C57Bl/6 mice were treated with four different doses of NFC (10, 40, 80 and 200 mu g/mouse), and samples were collected 24 h later. DNA damage was assessed by the comet assay in bronchoalveolar lavage (BAL) and lung cells, and chromosome damage by the bone marrow erythrocyte micronucleus assay. Inflammation was evaluated by BAL cell counts and analysis of cytokines and histopathological alterations in the lungs. A significant induction of DNA damage was observed at the two lower doses of NFC in lung cells, whereas no increase was seen in BAL cells. No effect was detected in the bone marrow micronucleus assay, either. NFC increased the recruitment of inflammatory cells to the lungs, together with a dose-dependent increase in mRNA expression of tumour necrosis factor a, interleukins 1 beta and 6, and chemokine (C-X-C motif) ligand 5, although there was no effect on the levels of the respective proteins. The histological analysis showed a dose-related accumulation of NFC in the bronchi, the alveoli and some in the cytoplasm of macrophages. In addition, neutrophilic accumulation in the alveolar lung space was observed with increasing dose. Our findings showed that NFC administered by pharyngeal aspiration caused an acute inflammatory response and DNA damage in the lungs, but no systemic genotoxic effect in the bone marrow. The present experimental design did not, however, allow us to determine whether the responses were transient or could persist for a longer time.
000075898 536__ $$9info:eu-repo/grantAgreement/EC/FP7/228802/EU/Scale-Up Nanoparticles in Modern Papermaking/SUNPAP
000075898 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000075898 590__ $$a2.84$$b2017
000075898 591__ $$aTOXICOLOGY$$b38 / 94 = 0.404$$c2017$$dQ2$$eT2
000075898 591__ $$aGENETICS & HEREDITY$$b78 / 171 = 0.456$$c2017$$dQ2$$eT2
000075898 592__ $$a0.916$$b2017
000075898 593__ $$aHealth, Toxicology and Mutagenesis$$c2017$$dQ2
000075898 593__ $$aToxicology$$c2017$$dQ2
000075898 593__ $$aGenetics$$c2017$$dQ3
000075898 593__ $$aGenetics (clinical)$$c2017$$dQ3
000075898 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000075898 700__ $$aRydman, E.
000075898 700__ $$aAimonen, K.
000075898 700__ $$aHannukainen, K.S.
000075898 700__ $$aSuhonen, S.
000075898 700__ $$aVanhala, E.
000075898 700__ $$0(orcid)0000-0002-1946-1187$$aMoreno, C.$$uUniversidad de Zaragoza
000075898 700__ $$aMeyer, V.
000075898 700__ $$aPerez, D.D.S.
000075898 700__ $$aSneck, A.
000075898 700__ $$aForsstrom, U.
000075898 700__ $$aHojgaard, C.
000075898 700__ $$aWillemoes, M.
000075898 700__ $$aWinther, J.R.
000075898 700__ $$aVogel, U.
000075898 700__ $$aWolff, H.
000075898 700__ $$aAlenius, H.
000075898 700__ $$aSavolainen, K.M.
000075898 700__ $$aNorppa, H.
000075898 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000075898 773__ $$g32, 1 (2017), 23-31$$pMutagenesis$$tMUTAGENESIS$$x0267-8357
000075898 8564_ $$s313887$$uhttps://zaguan.unizar.es/record/75898/files/texto_completo.pdf$$yPostprint
000075898 8564_ $$s103517$$uhttps://zaguan.unizar.es/record/75898/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000075898 909CO $$ooai:zaguan.unizar.es:75898$$particulos$$pdriver
000075898 951__ $$a2019-07-09-12:44:42
000075898 980__ $$aARTICLE