75969 20200716101511.0 doi 10.1016/j.jcis.2018.11.058 sideral 109106 ART-2019-109106 eng Armenia, I. Enzyme activation by alternating magnetic field: Importance of the bioconjugation methodology 2019 Access copy available to the general public Unrestricted Iron oxide nanoparticles (NPs) are attractive materials for enzyme immobilization and, thanks to their superparamagnetism, can be accessed by remote stimuli. This can be exploited to activate molecules that are not remotely actuable. Here, we demonstrate that thermophilic enzymes chemically linked to NPs can be activated in a “wireless” fashion by an external alternate magnetic field (AMF). To this aim, we have conjugated, with different binding strategies, the thermophilic enzymes a-amylase and L-aspartate oxidase to iron oxide NPs obtaining NP-enzyme systems with activities depending on the different orientations and stretching of the enzymes. Since enzyme activation occurs without a significant rise of the “overall” temperature of the systems, we have speculated a local NP-enzyme heating that does not immediately interest the rest of the solution that remains at relatively low temperature, low enough to allow non-thermophilic enzymes to work together with the NP-conjugated thermophilic enzymes. Nanoactuation of thermophilic enzymes by AMF has potential applications in different fields. Indeed, multi-enzymatic processes with enzymes with different temperature optima could be carried out in the same reaction pot and thermolabile products could be efficiently produced by thermophilic enzymes without suffering for the high temperatures. Moreover, our findings represent a proof of concept of the possibility to achieve a fine-tuning of the enzyme-NP system with the aim to intervene in cell metabolism. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 7.489 2019 CHEMISTRY, PHYSICAL 31 / 158 = 0.196 2019 Q1 T1 1.45 2019 Biomaterials 2019 Q1 Surfaces, Coatings and Films 2019 Q1 Electronic, Optical and Magnetic Materials 2019 Q1 Colloid and Surface Chemistry 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-6170-4237 Grazú Bonavia, M.V. Universidad de Zaragoza (orcid)0000-0001-6995-4302 De Matteis, L. Ivanchenko, P. Martra, G. Gornati, R. (orcid)0000-0003-1081-8482 de la Fuente, J.M. Bernardini, G. 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 537 (2019), 615-628 J. colloid interface sci. Journal of Colloid and Interface Science 0021-9797 453861 http://zaguan.unizar.es/record/75969/files/texto_completo.pdf Versión publicada 78047 http://zaguan.unizar.es/record/75969/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:75969 articulos driver 2020-07-16-09:18:53 ARTICLE