76038 20190709135455.0 doi 10.1016/j.anireprosci.2017.03.022 sideral 98976 ART-2017-98976 eng (orcid)0000-0001-5316-1703 Yániz, J.L. Universidad de Zaragoza Toward an integrative and predictive sperm quality analysis in Bos taurus 2017 Access copy available to the general public Unrestricted There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS®3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on membrane and acrosomal integrity, motility and morphology. It was also possible to observe live spermatozoa showing signs of capacitation such as hyperactivated motility and changes in acrosomal structure. Sperm subpopulation with intact plasma membrane and acrosome showed a higher proportion of motile sperm than those with damaged acrosome or increased fluorescence intensity. Spermatozoa with intact plasmalemma and damaged acrosome were static or exhibit weak movement. Significant correlations among the different sperm quality parameters evaluated were also described. We concluded that the ISAS®3Fun is an integrated method that represents an advance in sperm quality analysis with the potential to improve fertility predictions. info:eu-repo/grantAgreement/ES/DGA-FSE/A40 info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.647 2017 AGRICULTURE, DAIRY & ANIMAL SCIENCE 14 / 59 = 0.237 2017 Q1 T1 REPRODUCTIVE BIOLOGY 22 / 29 = 0.759 2017 Q4 T3 0.704 2017 Animal Science and Zoology 2017 Q1 Food Animals 2017 Q1 Medicine (miscellaneous) 2017 Q2 Endocrinology 2017 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Soler, C. (orcid)0000-0003-0389-2422 Alquézar-Baeta, C. Universidad de Zaragoza (orcid)0000-0001-8991-325X Santolaria, P. Universidad de Zaragoza 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 181 (2017), 108-114 Anim. reprod. sci. ANIMAL REPRODUCTION SCIENCE 0378-4320 501121 http://zaguan.unizar.es/record/76038/files/texto_completo.pdf Postprint 45066 http://zaguan.unizar.es/record/76038/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:76038 articulos driver 2019-07-09-11:43:04 ARTICLE