000076932 001__ 76932
000076932 005__ 20200117221624.0
000076932 0247_ $$2doi$$a10.1538/expanim.17-0152
000076932 0248_ $$2sideral$$a109839
000076932 037__ $$aART-2018-109839
000076932 041__ $$aeng
000076932 100__ $$aDomingo, P.
000076932 245__ $$aEffects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°c
000076932 260__ $$c2018
000076932 5060_ $$aAccess copy available to the general public$$fUnrestricted
000076932 5203_ $$aThe purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16°C for 72 h and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP. Each sample was then resuspended with extender A, B, C, or D, respectively. All samples were stored at 16°C and analysed at 4, 24, 48, and 72 h by ISAS®, vitality test, HOS test, and acrosome integrity test. After analyse of the results, SP samples showed a significantly higher percentage (P=0.020) in the HOS test (71.9 ± 1.6%) than non-SP samples (66.5 ± 1.6%). Non-SP samples had better results for kinematic parameters. Extenders A and C showed great results for the percentage of motile spermatozoa (63.1 ± 4.3% and 63.4 ± 3.7%, respectively), vitality (88.9 ± 2.6% and 87.7 ± 2.7%, respectively), and HOS test (68.9 ± 1.4% and 75.2 ± 1.4%, respectively). Extenders B and D showed worse data for sperm quality. These results suggest that SP has a protective effect on rabbit sperm membranes and maintains better sperm motility. The addition of glycerol and NMP to INRA does not improve rabbit sperm quality; nevertheless, the DMF cryoprotectant exerts a protective effect on the membrane of spermatozoa, improving seminal quality during rabbit sperm preservation at 16°C.
000076932 536__ $$9info:eu-repo/grantAgreement/ES/DGA/FSE
000076932 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000076932 590__ $$a1.208$$b2018
000076932 591__ $$aZOOLOGY$$b76 / 170 = 0.447$$c2018$$dQ2$$eT2
000076932 591__ $$aVETERINARY SCIENCES$$b54 / 141 = 0.383$$c2018$$dQ2$$eT2
000076932 592__ $$a0.5$$b2018
000076932 593__ $$aAnimal Science and Zoology$$c2018$$dQ2
000076932 593__ $$aVeterinary (miscellaneous)$$c2018$$dQ2
000076932 593__ $$aMedicine (miscellaneous)$$c2018$$dQ2
000076932 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2018$$dQ2
000076932 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000076932 700__ $$0(orcid)0000-0001-6479-3933$$aOlaciregui, M.
000076932 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, N.$$uUniversidad de Zaragoza
000076932 700__ $$0(orcid)0000-0002-1204-4356$$aDe Blas, I.$$uUniversidad de Zaragoza
000076932 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000076932 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000076932 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000076932 773__ $$g67, 4 (2018), 413-420$$pExp. anim.$$tEXPERIMENTAL ANIMALS$$x1341-1357
000076932 8564_ $$s772533$$uhttps://zaguan.unizar.es/record/76932/files/texto_completo.pdf$$yVersión publicada
000076932 8564_ $$s92091$$uhttps://zaguan.unizar.es/record/76932/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000076932 909CO $$ooai:zaguan.unizar.es:76932$$particulos$$pdriver
000076932 951__ $$a2020-01-17-21:55:59
000076932 980__ $$aARTICLE