76932 20200117221624.0 doi 10.1538/expanim.17-0152 sideral 109839 ART-2018-109839 eng Domingo, P. Effects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°c 2018 Access copy available to the general public Unrestricted The purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16°C for 72 h and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP. Each sample was then resuspended with extender A, B, C, or D, respectively. All samples were stored at 16°C and analysed at 4, 24, 48, and 72 h by ISAS®, vitality test, HOS test, and acrosome integrity test. After analyse of the results, SP samples showed a significantly higher percentage (P=0.020) in the HOS test (71.9 ± 1.6%) than non-SP samples (66.5 ± 1.6%). Non-SP samples had better results for kinematic parameters. Extenders A and C showed great results for the percentage of motile spermatozoa (63.1 ± 4.3% and 63.4 ± 3.7%, respectively), vitality (88.9 ± 2.6% and 87.7 ± 2.7%, respectively), and HOS test (68.9 ± 1.4% and 75.2 ± 1.4%, respectively). Extenders B and D showed worse data for sperm quality. These results suggest that SP has a protective effect on rabbit sperm membranes and maintains better sperm motility. The addition of glycerol and NMP to INRA does not improve rabbit sperm quality; nevertheless, the DMF cryoprotectant exerts a protective effect on the membrane of spermatozoa, improving seminal quality during rabbit sperm preservation at 16°C. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.208 2018 ZOOLOGY 76 / 170 = 0.447 2018 Q2 T2 VETERINARY SCIENCES 54 / 141 = 0.383 2018 Q2 T2 0.5 2018 Animal Science and Zoology 2018 Q2 Veterinary (miscellaneous) 2018 Q2 Medicine (miscellaneous) 2018 Q2 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-6479-3933 Olaciregui, M. (orcid)0000-0002-1611-6337 González, N. Universidad de Zaragoza (orcid)0000-0002-1204-4356 De Blas, I. Universidad de Zaragoza (orcid)0000-0002-0797-1841 Gil, L. Universidad de Zaragoza 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 67, 4 (2018), 413-420 Exp. anim. EXPERIMENTAL ANIMALS 1341-1357 772533 http://zaguan.unizar.es/record/76932/files/texto_completo.pdf Versión publicada 92091 http://zaguan.unizar.es/record/76932/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:76932 articulos driver 2020-01-17-21:55:59 ARTICLE