000076970 001__ 76970 000076970 005__ 20200117221624.0 000076970 0247_ $$2doi$$a10.1103/PhysRevE.98.062501 000076970 0248_ $$2sideral$$a109858 000076970 037__ $$aART-2018-109858 000076970 041__ $$aeng 000076970 100__ $$0(orcid)0000-0001-6859-099X$$aFiasconaro, A.$$uUniversidad de Zaragoza 000076970 245__ $$aForce spectroscopy analysis in polymer translocation 000076970 260__ $$c2018 000076970 5060_ $$aAccess copy available to the general public$$fUnrestricted 000076970 5203_ $$aThis paper reports the force spectroscopy analysis of a polymer that translocates from one side of a membrane to the other side through an extended pore, pulled by a cantilever that moves with constant velocity against the damping and the potential barrier generated by the reaction of the membrane walls. The polymer is modeled as a beads-springs chain with both excluded volume and bending contributions and moves in a stochastic three-dimensional environment described by a Langevin dynamics at fixed temperature. The force trajectories recorded at different velocities reveal two exponential regimes: The force increases when the first part of the chain enters the pore and then decreases when the first monomer reaches the trans region. The spectroscopy analysis of the force values permit the estimation of the limit force to allow the translocation, related to the free-energy barrier. The stall force to maintain the polymer fixed has been also calculated independently, and its value confirms the force spectroscopy outcomes. 000076970 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E19$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2014-55867-P$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2017-87519-P 000076970 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000076970 590__ $$a2.353$$b2018 000076970 591__ $$aPHYSICS, MATHEMATICAL$$b7 / 55 = 0.127$$c2018$$dQ1$$eT1 000076970 591__ $$aPHYSICS, FLUIDS & PLASMAS$$b14 / 32 = 0.438$$c2018$$dQ2$$eT2 000076970 592__ $$a0.992$$b2018 000076970 593__ $$aCondensed Matter Physics$$c2018$$dQ1 000076970 593__ $$aStatistics and Probability$$c2018$$dQ1 000076970 593__ $$aStatistical and Nonlinear Physics$$c2018$$dQ1 000076970 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000076970 700__ $$0(orcid)0000-0002-9551-624X$$aFalo, F.$$uUniversidad de Zaragoza 000076970 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada 000076970 773__ $$g98, 6 (2018), 062501 [7 pp]$$pPhys. rev., E$$tPhysical Review E$$x2470-0045 000076970 8564_ $$s695546$$uhttps://zaguan.unizar.es/record/76970/files/texto_completo.pdf$$yVersión publicada 000076970 8564_ $$s18400$$uhttps://zaguan.unizar.es/record/76970/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000076970 909CO $$ooai:zaguan.unizar.es:76970$$particulos$$pdriver 000076970 951__ $$a2020-01-17-21:56:03 000076970 980__ $$aARTICLE