76970 20200117221624.0 doi 10.1103/PhysRevE.98.062501 sideral 109858 ART-2018-109858 eng (orcid)0000-0001-6859-099X Fiasconaro, A. Universidad de Zaragoza Force spectroscopy analysis in polymer translocation 2018 Access copy available to the general public Unrestricted This paper reports the force spectroscopy analysis of a polymer that translocates from one side of a membrane to the other side through an extended pore, pulled by a cantilever that moves with constant velocity against the damping and the potential barrier generated by the reaction of the membrane walls. The polymer is modeled as a beads-springs chain with both excluded volume and bending contributions and moves in a stochastic three-dimensional environment described by a Langevin dynamics at fixed temperature. The force trajectories recorded at different velocities reveal two exponential regimes: The force increases when the first part of the chain enters the pore and then decreases when the first monomer reaches the trans region. The spectroscopy analysis of the force values permit the estimation of the limit force to allow the translocation, related to the free-energy barrier. The stall force to maintain the polymer fixed has been also calculated independently, and its value confirms the force spectroscopy outcomes. info:eu-repo/grantAgreement/ES/DGA/E19 info:eu-repo/grantAgreement/ES/MINECO/FIS2014-55867-P info:eu-repo/grantAgreement/ES/MINECO/FIS2017-87519-P info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.353 2018 PHYSICS, MATHEMATICAL 7 / 55 = 0.127 2018 Q1 T1 PHYSICS, FLUIDS & PLASMAS 14 / 32 = 0.438 2018 Q2 T2 0.992 2018 Condensed Matter Physics 2018 Q1 Statistics and Probability 2018 Q1 Statistical and Nonlinear Physics 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-9551-624X Falo, F. Universidad de Zaragoza 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 98, 6 (2018), 062501 [7 pp] Phys. rev., E Physical Review E 2470-0045 695546 http://zaguan.unizar.es/record/76970/files/texto_completo.pdf Versión publicada 18400 http://zaguan.unizar.es/record/76970/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:76970 articulos driver 2020-01-17-21:56:03 ARTICLE