76976 20200117221625.0 doi 10.1038/s41598-018-35243-4 sideral 109867 ART-2018-109867 eng Moen, A.E.F. The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients 2018 Access copy available to the general public Unrestricted Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-naïve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls. info:eu-repo/grantAgreement/EC/FP7/305676/EU/Inflammatory Bowel Disease CHARACTERization by a multi-modal integrated biomarker study/IBD-CHARACTER info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.011 2018 MULTIDISCIPLINARY SCIENCES 14 / 69 = 0.203 2018 Q1 T1 1.414 2018 Multidisciplinary 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lindstrøm, J.C. Tannæs, T.M. Vatn, S. Ricanek, P. Vatn, M.H. Jahnsen, J. Frengen, A.B. Dahl, F.A. You, P. Sølvernes, J. Ekeland, G.S. Detlie, T.E. Olbjørn, C. O’Leary, K.R. Ventham, N.T. Kennedy, N.A. Kalla, R. Adams, A. Drummond, H.E. Boyapati, R. Nimmo, E.R. Wilson, D.C. Satsangi, J. Heath, S.C. Gut, M. Merkel, A. Bayes, M. Gut, I.G. Keita, Å.V. Söderholm, J.D. Hjortswang, H. Carstens, A. Bergemalm, D. Halfvarson, J. Andersson, E. Lindqvist, M. Repsilber, D. Pierik, M. Jonkers, D. Gomollón, F. D’Amato, M. Törkvist, L. Hjelm, F. Gullberg, M. Nordberg, N. Ocklind, A. Pettersson, E. Ekman, D. Sundell, M. Modig, E. Bonfiglio, F. Veillard, A.C. Schoemans, R. (orcid)0000-0003-0076-3529 Poncelet, D. Universidad de Zaragoza Sabatel, C. Lindahl, T. Ciemniejewska, E. Casén, C. Lees, C. Noble, C.L. Arnott, I. Ho, G.T. Shand, A.G. The, IBD-Character, Consortium 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 8, 1 (2018), 17278 [12 pp] Sci. rep. SCIENTIFIC REPORTS 2045-2322 1722718 http://zaguan.unizar.es/record/76976/files/texto_completo.pdf Versión publicada 110918 http://zaguan.unizar.es/record/76976/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:76976 articulos driver 2020-01-17-21:56:40 ARTICLE