000076984 001__ 76984
000076984 005__ 20191212100704.0
000076984 0247_ $$2doi$$a10.1016/j.cryobiol.2018.01.004
000076984 0248_ $$2sideral$$a105850
000076984 037__ $$aART-2018-105850
000076984 041__ $$aeng
000076984 100__ $$aDomingo, P.
000076984 245__ $$aLong-term preservation of freeze-dried rabbit sperm by adding rosmarinic acid and different chelating agents
000076984 260__ $$c2018
000076984 5060_ $$aAccess copy available to the general public$$fUnrestricted
000076984 5203_ $$aFreeze-drying (FD) technique has been applied as an alternative technology to preserve gene resources to allow simple sperm preservation and shipment at 4 degrees C. Nevertheless, DNA sperm might be damaged by mechanical or oxidative stress throughout FD procedure. Therefore, suitable protection to maintain DNA integrity is required. The aim of this study was to determine the effect of rosmarinic acid (RA) as an antioxidant and two chelating agents (EGTA and EDTA) on the DNA integrity of freeze-dried rabbit sperm after storage of the samples at 4 degrees C and room temperature for 8 months. Rabbit sperm were freeze-dried in basic medium (10 mM Tris-HCl buffer and 50 mM NaCl) supplemented with 50 mM EGTA (1), 50 mM EGTA plus 105 mu M RA (2), 50 mM EDTA (3) or 50 mM EDTA plus 105 mu M RA (4). Semen samples were kept at 4 degrees C and room temperature during 8 months. After rehydration, DNA integrity was evaluated with Sperm Chromatin Dispersion test observing that DNA fragmentation was higher when semen samples were freeze-dried with EGTA (10.9%) than with EDTA (4.1%) (p < 0.01). Furthermore, RA acted better under adverse conditions and no significant differences were found in temperature storage. Summarizing, FD is a method that can allow simple gene resources preservation among 4 degrees C to 25 degrees C during 8 months and transportation without the need for liquid nitrogen or dry ice. EDTA chelating agent is the most suitable media for freeze-dried rabbit sperm and the addition of RA protects the DNA against the oxidative stress caused during FD procedure.
000076984 536__ $$9info:eu-repo/grantAgreement/ES/DGA/FSE
000076984 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000076984 590__ $$a2.141$$b2018
000076984 591__ $$aBIOLOGY$$b29 / 87 = 0.333$$c2018$$dQ2$$eT2
000076984 591__ $$aPHYSIOLOGY$$b51 / 81 = 0.63$$c2018$$dQ3$$eT2
000076984 592__ $$a0.711$$b2018
000076984 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2018$$dQ1
000076984 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000076984 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2018$$dQ1
000076984 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000076984 700__ $$0(orcid)0000-0001-6479-3933$$aOlaciregui, M.
000076984 700__ $$0(orcid)0000-0002-1611-6337$$aGonzalez, N.$$uUniversidad de Zaragoza
000076984 700__ $$0(orcid)0000-0002-1204-4356$$aDe Blas, I.$$uUniversidad de Zaragoza
000076984 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000076984 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000076984 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000076984 773__ $$g81 (2018), 174-177$$pCryobiology$$tCryobiology$$x0011-2240
000076984 8564_ $$s188499$$uhttps://zaguan.unizar.es/record/76984/files/texto_completo.pdf$$yPostprint
000076984 8564_ $$s95274$$uhttps://zaguan.unizar.es/record/76984/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000076984 909CO $$ooai:zaguan.unizar.es:76984$$particulos$$pdriver
000076984 951__ $$a2019-12-12-10:05:57
000076984 980__ $$aARTICLE