Liver bioengineering using decellularized whole-liver scaffolds

Pla-Palacín, I. ; Sainz-Arnal, P. ; Morini, S. ; Almeida, M. ; Baptista, P.M.

Resumen: Currently, due to the progress made in the field of regenerative medicine, whole-organ bioengineering is becoming a valid alternative to cope with the shortages of organs for transplantation. In this chapter, we describe the main techniques carried out for pig liver bioengineering, which serves as an essential model for future human liver bioengineering. These include porcine whole-liver decellularization, endothelial and mesenchymal stem cell isolation, porcine ES-derived hepatoblasts, and scaffold recellularization using a bioreactor perfusion system.

Idioma: Inglés

DOI: 10.1007/7651_2017_98

Año: 2018

Publicado en: Methods in Molecular Biology 1577 (2018), 293-305
ISSN: 1064-3745

Factor impacto SCIMAGO: 0.605 - Molecular Biology (Q3) - Genetics (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA/C066-2014
Financiación: info:eu-repo/grantAgreement/EC/H2020/657566/EU/Controlling Cell Fate through Smart Nanoheaters/CONFINES
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-00563
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/i-PFIS-IFI15-00158

Tipo y forma: Artículo (PostPrint)