000077125 001__ 77125
000077125 005__ 20190709135606.0
000077125 0247_ $$2doi$$a10.1038/s41598-017-08958-z
000077125 0248_ $$2sideral$$a108241
000077125 037__ $$aART-2017-108241
000077125 041__ $$aeng
000077125 100__ $$0(orcid)0000-0002-5895-2157$$aGayán, Elisa
000077125 245__ $$aRpoS-independent evolution reveals the importance of attenuated cAMPCRP regulation in high hydrostatic pressure resistance acquisition in E. coli
000077125 260__ $$c2017
000077125 5060_ $$aAccess copy available to the general public$$fUnrestricted
000077125 5203_ $$aHigh hydrostatic pressure (HHP) processing is an attractive non-thermal alternative to food pasteurization. Nevertheless, the large inter- and intra-species variations in HHP resistance among foodborne pathogens and the ease by which they can acquire extreme resistance are an issue of increasing concern. Since RpoS activity has been considered as a central determinant in the HHP resistance of E. coli and its pathovars, this study probed for the potential of an E. coli MG1655 ΔrpoS mutant to acquire HHP resistance by directed evolution. Despite the higher initial HHP sensitivity of the ΔrpoS mutant compared to the wild-type strain, evolved lineages of the former readily managed to restore or even succeed wild-type levels of resistance. A number of these ΔrpoS derivatives were affected in cAMP/CRP regulation, and this could be causally related to their HHP resistance. Subsequent inspection revealed that some of previously isolated HHP-resistant mutants derived from the wild-type strain also incurred a causal decrease in cAMP/CRP regulation. cAMP/CRP attenuated HHP-resistant mutants also exhibited higher resistance to fosfomycin, a preferred treatment for STEC infections. As such, this study reveals attenuation of cAMP/CRP regulation as a relevant and RpoS-independent evolutionary route towards HHP resistance in E. coli that coincides with fosfomycin resistance.
000077125 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000077125 590__ $$a4.122$$b2017
000077125 591__ $$aMULTIDISCIPLINARY SCIENCES$$b12 / 64 = 0.188$$c2017$$dQ1$$eT1
000077125 592__ $$a1.533$$b2017
000077125 593__ $$aMultidisciplinary$$c2017$$dQ1
000077125 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000077125 700__ $$aCambré, Alexander
000077125 700__ $$aMichiels, Chris W.
000077125 700__ $$aAertsen, Abram
000077125 773__ $$g7 (2017), 8600 [11 pp.]$$pSci. rep.$$tScientific reports$$x2045-2322
000077125 8564_ $$s1592640$$uhttps://zaguan.unizar.es/record/77125/files/texto_completo.pdf$$yVersión publicada
000077125 8564_ $$s108833$$uhttps://zaguan.unizar.es/record/77125/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000077125 909CO $$ooai:zaguan.unizar.es:77125$$particulos$$pdriver
000077125 951__ $$a2019-07-09-12:20:12
000077125 980__ $$aARTICLE