000077145 001__ 77145
000077145 005__ 20240123082600.0
000077145 0247_ $$2doi$$a10.1177/0300985818809142
000077145 0248_ $$2sideral$$a110339
000077145 037__ $$aART-2018-110339
000077145 041__ $$aeng
000077145 100__ $$0(orcid)0000-0002-6178-4801$$aAsín, J.$$uUniversidad de Zaragoza
000077145 245__ $$aGranulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep
000077145 260__ $$c2018
000077145 5060_ $$aAccess copy available to the general public$$fUnrestricted
000077145 5203_ $$aThe use of vaccines including aluminum (Al)–based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher (P <.001) in the lymph nodes of the vaccine group (82.65 µg/g) compared with both adjuvant-only (2.53 µg/g) and control groups (0.96 µg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs.
000077145 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-2-R
000077145 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000077145 590__ $$a2.116$$b2018
000077145 591__ $$aVETERINARY SCIENCES$$b14 / 141 = 0.099$$c2018$$dQ1$$eT1
000077145 591__ $$aPATHOLOGY$$b36 / 76 = 0.474$$c2018$$dQ2$$eT2
000077145 592__ $$a0.893$$b2018
000077145 593__ $$aVeterinary (miscellaneous)$$c2018$$dQ1
000077145 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000077145 700__ $$0(orcid)0000-0001-5050-0167$$aMolín, J.$$uUniversidad de Zaragoza
000077145 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, M.$$uUniversidad de Zaragoza
000077145 700__ $$0(orcid)0000-0002-9210-6026$$aPinczowski, P.
000077145 700__ $$aGimeno, M.
000077145 700__ $$0(orcid)0000-0003-3363-2912$$aNavascués, N.
000077145 700__ $$0(orcid)0000-0003-3074-5778$$aMuniesa, A.$$uUniversidad de Zaragoza
000077145 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, I.$$uUniversidad de Zaragoza
000077145 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, D.$$uUniversidad de Zaragoza
000077145 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, A.$$uUniversidad de Zaragoza
000077145 700__ $$ade Pablo, L.
000077145 700__ $$aMold, M.
000077145 700__ $$aExley, C.
000077145 700__ $$ade Andrés, D.
000077145 700__ $$aReina, R.
000077145 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, L.$$uUniversidad de Zaragoza
000077145 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000077145 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000077145 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000077145 773__ $$g56, 3 (2018), 418-428$$pVet. pathol.$$tVeterinary Pathology$$x0300-9858
000077145 8564_ $$s651095$$uhttps://zaguan.unizar.es/record/77145/files/texto_completo.pdf$$yPostprint
000077145 8564_ $$s110172$$uhttps://zaguan.unizar.es/record/77145/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000077145 909CO $$ooai:zaguan.unizar.es:77145$$particulos$$pdriver
000077145 951__ $$a2024-01-23-08:21:00
000077145 980__ $$aARTICLE