77145 20240123082600.0 doi 10.1177/0300985818809142 sideral 110339 ART-2018-110339 eng Asín, J. Universidad de Zaragoza (orcid)0000-0002-6178-4801 Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep 2018 Access copy available to the general public Unrestricted The use of vaccines including aluminum (Al)–based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher (P <.001) in the lymph nodes of the vaccine group (82.65 µg/g) compared with both adjuvant-only (2.53 µg/g) and control groups (0.96 µg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs. info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-1-R info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-2-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.116 2018 VETERINARY SCIENCES 14 / 141 = 0.099 2018 Q1 T1 PATHOLOGY 36 / 76 = 0.474 2018 Q2 T2 0.893 2018 Veterinary (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Molín, J. Universidad de Zaragoza (orcid)0000-0001-5050-0167 Pérez, M. Universidad de Zaragoza (orcid)0000-0002-8133-2124 Pinczowski, P. (orcid)0000-0002-9210-6026 Gimeno, M. Navascués, N. (orcid)0000-0003-3363-2912 Muniesa, A. Universidad de Zaragoza (orcid)0000-0003-3074-5778 de Blas, I. Universidad de Zaragoza (orcid)0000-0002-1204-4356 Lacasta, D. Universidad de Zaragoza (orcid)0000-0002-7822-6646 Fernández, A. Universidad de Zaragoza (orcid)0000-0002-2557-4890 de Pablo, L. Mold, M. Exley, C. de Andrés, D. Reina, R. Luján, L. Universidad de Zaragoza (orcid)0000-0002-2053-9842 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 56, 3 (2018), 418-428 Vet. pathol. Veterinary Pathology 0300-9858 651095 http://zaguan.unizar.es/record/77145/files/texto_completo.pdf Postprint 110172 http://zaguan.unizar.es/record/77145/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:77145 articulos driver 2024-01-23-08:21:00 ARTICLE