77170 20210108084705.0 doi 10.1186/s12951-018-0437-z sideral 110377 ART-2019-110377 eng (orcid)0000-0001-8762-5457 Sancho-Albero, M. Universidad de Zaragoza Exosome origin determines cell targeting and the transfer of therapeutic nanoparticles towards target cells 2019 Access copy available to the general public Unrestricted Background: Exosomes are considered key elements for communication between cells, but very little is known about the mechanisms and selectivity of the transference processes involving exosomes released from different cells. Results: In this study we have investigated the transfer of hollow gold nanoparticles (HGNs) between different cells when these HGNs were loaded within exosomes secreted by human placental mesenchymal stem cells (MSCs). These HGNs were successfully incorporated in the MSCs exosome biogenesis pathway and released as HGNs-loaded exosomes. Time-lapse microscopy and atomic emission spectroscopy allowed us to demonstrate the selective transfer of the secreted exosomes only to the cell type of origin when studying different cell types including cancer, metastatic, stem or immunological cells. Conclusions: In this study we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Specifically, we show how this preferential uptake can be leveraged to selectively induce cell death by light-induced hyperthermia only in cells of the same type as those producing the corresponding loaded exosomes. We describe how the exosomes are preferentially transferred to some cell types but not to others, thus providing a better understanding to design selective therapies for different diseases.[Figure not available: see fulltext.] info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.518 2019 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 15 / 156 = 0.096 2019 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 30 / 103 = 0.291 2019 Q2 T1 1.335 2019 Applied Microbiology and Biotechnology 2019 Q1 Bioengineering 2019 Q1 Biomedical Engineering 2019 Q1 Pharmaceutical Science 2019 Q1 Medicine (miscellaneous) 2019 Q1 Nanoscience and Nanotechnology 2019 Q2 Molecular Medicine 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0003-3363-2912 Navascués, N. (orcid)0000-0003-2293-363X Mendoza, G. Universidad de Zaragoza (orcid)0000-0002-6873-5244 Sebastián, V. Universidad de Zaragoza (orcid)0000-0003-3165-0156 Arruebo, M. Universidad de Zaragoza (orcid)0000-0003-4160-9720 Martín-Duque, P. (orcid)0000-0002-8701-9745 Santamaría, J. Universidad de Zaragoza 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 17, 1 (2019), 16 [13 pp] J. nanobiotechnol. Journal of Nanobiotechnology 1477-3155 1725510 http://zaguan.unizar.es/record/77170/files/texto_completo.pdf Versión publicada 63975 http://zaguan.unizar.es/record/77170/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:77170 articulos driver 2021-01-08-08:04:02 ARTICLE