Gold nanoparticle coatings as efficient adenovirus carriers to non-infectable stem cells

Hernandez, Y. ; González-Pastor, R. ; Belmar-Lopez, C. ; Mendoza, G. (Universidad de Zaragoza) ; De La Fuente, J.M. ; Martin-Duque, P.

Resumen: Mesenchymal stem cells (MSCs) are adult pluripotent cells with the plasticity to be converted into different cell types. Their self-renewal capacity, relative ease of isolation, expansion and inherent migration to tumors, make them perfect candidates for cell therapy against cancer. However, MSCs are notoriously refractory to adenoviral infection, mainly because CAR (Coxsackie-Adenovirus Receptor) expression is absent or downregulated. Over the last years, nanoparticles have attracted a great deal of attention as potential vehicle candidates for gene delivery, but with limited effects on their own. Our data showed that the use of positively charged 14 nm gold nanoparticles either functionalized with arginine-glycine-aspartate (RGD) motif or not, increases the efficiency of adenovirus infection in comparison to commercial reagents without altering cell viability or cell phenotype. This system represents a simple, efficient and safe method for the transduction of MSCs, being attractive for cancer gene and cell therapies.

Idioma: Inglés

DOI: 10.1039/C8RA09088B

Año: 2019

Publicado en: RSC Advances 9, 3 (2019), 1327-1334
ISSN: 2046-2069

Factor impacto JCR: 3.119 (2019)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 73 / 177 = 0.412 (2019) - Q2 - T2

Factor impacto SCIMAGO: 0.736 - Chemistry (miscellaneous) (Q1) - Chemical Engineering (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B054-12
Financiación: info:eu-repo/grantAgreement/EC/FP7/219433/EU/Transnational call for collaborative proposals in basic nanoscience research/NANOSCI-EPLUS

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)