000077266 001__ 77266
000077266 005__ 20200113145618.0
000077266 0247_ $$2doi$$a10.1021/acssynbio.7b00383
000077266 0248_ $$2sideral$$a106542
000077266 037__ $$aART-2018-106542
000077266 041__ $$aeng
000077266 100__ $$aBenítez-Mateos, A.I.
000077266 245__ $$aExpanding one-pot cell-free protein synthesis and immobilization for on-demand manufacturing of biomaterials
000077266 260__ $$c2018
000077266 5060_ $$aAccess copy available to the general public$$fUnrestricted
000077266 5203_ $$aFabrication of protein-based biomaterials is an arduous and time-consuming procedure with multiple steps. In this work, we describe a portable toolkit that integrates both cell-free protein synthesis (CFPS) and protein immobilization in one pot just by mixing DNA, solid materials, and a CFPS system. We have constructed a modular set of plasmids that fuse the N-terminus of superfolded green fluorescent protein (sGFP) with different peptide tags (poly(6X)Cys, poly(6X)His, and poly(6X)Lys), which drive the immobilization of the protein on the tailored material (agarose beads with different functionalities, gold nanorods, and silica nanoparticles). This system also enables the incorporation of azide-based amino acids into the nascent protein for its selective immobilization through copper-free click reactions. Finally, this technology has been expanded to the synthesis and immobilization of enzymes and antibody-binding proteins for the fabrication of functional biomaterials. This synthetic biological platform has emerged as a versatile tool for on-demand fabrication of therapeutic, diagnostic, and sensing biomaterials.
000077266 536__ $$9info:eu-repo/grantAgreement/EUR/COST/CM1303$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP
000077266 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000077266 590__ $$a5.571$$b2018
000077266 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b6 / 78 = 0.077$$c2018$$dQ1$$eT1
000077266 592__ $$a2.722$$b2018
000077266 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2018$$dQ1
000077266 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000077266 593__ $$aBiomedical Engineering$$c2018$$dQ1
000077266 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000077266 700__ $$aLlarena, I.
000077266 700__ $$aSánchez-Iglesias, A.
000077266 700__ $$0(orcid)0000-0003-0031-1880$$aLópez-Gallego, F.$$uUniversidad de Zaragoza
000077266 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000077266 773__ $$g7, 3 (2018), 875-884$$pACS synthetic biology$$tACS Synthetic Biology$$x2161-5063
000077266 8564_ $$s1171226$$uhttps://zaguan.unizar.es/record/77266/files/texto_completo.pdf$$yPostprint
000077266 8564_ $$s62791$$uhttps://zaguan.unizar.es/record/77266/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000077266 909CO $$ooai:zaguan.unizar.es:77266$$particulos$$pdriver
000077266 951__ $$a2020-01-13-14:54:17
000077266 980__ $$aARTICLE