77266 20200113145618.0 doi 10.1021/acssynbio.7b00383 sideral 106542 ART-2018-106542 eng Benítez-Mateos, A.I. Expanding one-pot cell-free protein synthesis and immobilization for on-demand manufacturing of biomaterials 2018 Access copy available to the general public Unrestricted Fabrication of protein-based biomaterials is an arduous and time-consuming procedure with multiple steps. In this work, we describe a portable toolkit that integrates both cell-free protein synthesis (CFPS) and protein immobilization in one pot just by mixing DNA, solid materials, and a CFPS system. We have constructed a modular set of plasmids that fuse the N-terminus of superfolded green fluorescent protein (sGFP) with different peptide tags (poly(6X)Cys, poly(6X)His, and poly(6X)Lys), which drive the immobilization of the protein on the tailored material (agarose beads with different functionalities, gold nanorods, and silica nanoparticles). This system also enables the incorporation of azide-based amino acids into the nascent protein for its selective immobilization through copper-free click reactions. Finally, this technology has been expanded to the synthesis and immobilization of enzymes and antibody-binding proteins for the fabrication of functional biomaterials. This synthetic biological platform has emerged as a versatile tool for on-demand fabrication of therapeutic, diagnostic, and sensing biomaterials. info:eu-repo/grantAgreement/EUR/COST/CM1303 info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.571 2018 BIOCHEMICAL RESEARCH METHODS 6 / 78 = 0.077 2018 Q1 T1 2.722 2018 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2018 Q1 Medicine (miscellaneous) 2018 Q1 Biomedical Engineering 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Llarena, I. Sánchez-Iglesias, A. (orcid)0000-0003-0031-1880 López-Gallego, F. Universidad de Zaragoza 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 7, 3 (2018), 875-884 ACS synthetic biology ACS Synthetic Biology 2161-5063 1171226 http://zaguan.unizar.es/record/77266/files/texto_completo.pdf Postprint 62791 http://zaguan.unizar.es/record/77266/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:77266 articulos driver 2020-01-13-14:54:17 ARTICLE