000077600 001__ 77600 000077600 005__ 20190226114802.0 000077600 037__ $$aTAZ-TFG-2018-2893 000077600 041__ $$aeng 000077600 1001_ $$aTorres Herrero, Beatriz 000077600 24200 $$aCharacterization of Klebsiella pneumoniae bacteriophages with biotechnological potencial 000077600 24500 $$aCaracterización de los fagos de Klebsiella pneumoniae con potencial biotecnológico 000077600 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2018 000077600 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000077600 520__ $$aThe extensive use and misuse of antibiotics has led to an increased emergence of multidrug-resistant Klebsiella pneumoniae strains. They are a serious concern worldwide due to their propensity to spread and the scarce effective treatments left. Consequently, phage therapy is garnering renewed interest as an alternative method to defeat antibiotic resistant bacteria. Phages – natural pathogens of bacteria – have several properties: high capacity to replicate and host specificity that turns them into a great advantage over antibiotics. Eight bacteriophages infecting Klebsiella pneumoniae were characterized according to their genetic material and morphology by performing endonuclease digestions and transmission electron microscopy imaging with 1% phosphotungstic acid or 2% uranyl acetate as staining dyes. Then, they were classified in agreement with their morphological characterization. Seven phages (EKP3P1, EKP3P2, EKP3P4, EKP3P5, EKP8P2, EKP8P3 and EKP8P4) were classified into Siphoviridae family showing hexagonal heads with long non- contractile, sometimes flexible tails and closely related restriction patterns. EKP8P1 phage was classified into Podoviridae family showing an icosahedral head with a short non-contractile tail and a different restriction pattern. They all belong to Caudovirales order. Moreover, a prophage was found in EKP8P1 sample, and classified into Siphoviridae family according to its morphology. The genome of EKP3P5 phage, a double stranded DNA of 47,622 bp long, was sequenced and annotated manually. EKP3P5 phage is a temperate phage encoding integrase, holin and endolysin proteins, among others. Therefore, EKP3P5 could not be used in phage therapy due to the risk of transferring virulence and resistance genes to the host bacteria. For all the above reasons, this thesis provides detailed knowledge of the physical structure along with genomic qualities of eight bacteriophages infecting multidrug- resistant Klebsiella pneumoniae strains. This is important for determining the potential of phages as therapeutic agents and the first step to improve phage therapy. 000077600 521__ $$aGraduado en Biotecnología 000077600 540__ $$aDerechos regulados por licencia Creative Commons 000077600 700__ $$aJalasvuori, Matti$$edir. 000077600 700__ $$aPenttinen, Reetta$$edir. 000077600 7102_ $$aUniversidad de Zaragoza$$bMicrobiología, Medicina Preventiva y Salud Pública$$cMicrobiología 000077600 7202_ $$aGonzalo Asensio, Jesús$$eponente 000077600 8560_ $$f698536@celes.unizar.es 000077600 8564_ $$s1547385$$uhttps://zaguan.unizar.es/record/77600/files/TAZ-TFG-2018-2893.pdf$$yMemoria (eng) 000077600 8564_ $$s158101$$uhttps://zaguan.unizar.es/record/77600/files/TAZ-TFG-2018-2893_ANE.pdf$$yAnexos (eng) 000077600 909CO $$ooai:zaguan.unizar.es:77600$$pdriver$$ptrabajos-fin-grado 000077600 950__ $$a 000077600 951__ $$adeposita:2019-02-26 000077600 980__ $$aTAZ$$bTFG$$cCIEN 000077600 999__ $$a20180830102407.CREATION_DATE