000077678 001__ 77678 000077678 005__ 20190226114805.0 000077678 037__ $$aTAZ-TFG-2018-2578 000077678 041__ $$aeng 000077678 1001_ $$aCastillón Clemente, Leticia 000077678 24200 $$aIdentification and characterization of natural yeasts in palm wine 000077678 24500 $$aIdentification and characterization of natural yeasts in palm wine 000077678 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2018 000077678 500__ $$aLos resultados del proyecto están incluidos en el anexo. 000077678 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000077678 520__ $$aIn this project, I identified the yeast species from over 1000 samples originating from Nigerian palm wine, using a combined protocol of PCR amplification, restriction fragment length polymorfism (RFLP) and multiplex PCR. Most of the identified samples correspond to Saccharomyces cerevisiae, usually main yeast participating in fermentation processes, which makes sense with the origin of the studied material. However, for some other results different data was obtained, which could correspond to different Saccharomyces sp. as Saccharomyces mikatae, Saccharomyces arboricola or Saccharomyces paradoxus, species that had not been previously identified in Africa. These unexpected results should be contrasted with the data obtained from the phenotyping analysis and, since the only way to unambiguously identify a species is by sequencing its DNA, the sequencing of the ITS region of a subset of the strains that seem to belong to one of the species mentioned above would be mandatory to confirm the finding. 000077678 521__ $$aGraduado en Biotecnología 000077678 540__ $$aDerechos regulados por licencia Creative Commons 000077678 700__ $$aWarringer, Jonas$$edir. 000077678 700__ $$aWarringer, Jonas$$edir. 000077678 7102_ $$aUniversidad de Zaragoza$$bBioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular 000077678 8560_ $$f698034@celes.unizar.es 000077678 8564_ $$s172285$$uhttps://zaguan.unizar.es/record/77678/files/TAZ-TFG-2018-2578_ANE.pdf$$yAnexos (eng) 000077678 8564_ $$s2708377$$uhttps://zaguan.unizar.es/record/77678/files/TAZ-TFG-2018-2578.pdf$$yMemoria (eng) 000077678 909CO $$ooai:zaguan.unizar.es:77678$$pdriver$$ptrabajos-fin-grado 000077678 950__ $$a 000077678 951__ $$adeposita:2019-02-26 000077678 980__ $$aTAZ$$bTFG$$cCIEN 000077678 999__ $$a20180629115318.CREATION_DATE