78020 20250313085413.0 doi 10.2217/nnm-2018-0258 sideral 110570 ART-2019-110570 eng Andreu, Vanesa (orcid)0000-0002-6275-1104 Matryoshka-type gastro-resistant microparticles for the oral treatment of Mycobacterium tuberculosis 2019 Access copy available to the general public Unrestricted Aim: Production of Matryoshka-type gastroresistant microparticles containing antibiotic-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NP) against Mycobacterium tuberculosis. Materials & methods: The emulsification and evaporation methods were followed for the synthesis of PLGA–NPs and methacrylic acid-ethyl acrylate-based coatings to protect rifampicin from degradation under simulated gastric conditions. Results & conclusion: The inner antibiotic-loaded NPs here reported can be released under simulated intestinal conditions whereas their coating protects them from degradation under simulated gastric conditions. The encapsulation does not hinder the antituberculosis action of the encapsulated antibiotic rifampicin. A sustained antibiotic release could be obtained when using the drug-loaded encapsulated NPs. Compared with the administration of the free drug, a more effective elimination of M. tuberculosis was observed when applying the NPs against infected macrophages. The antibiotic-loaded PLGA–NPs were also able to cross an in vitro model of intestinal barrier. Matryoshka-type gastroresistant microparticles containing antibiotic-loaded poly lactic-co-glycolic acid nanoparticles against M. tuberculosis were produced to protect the antibiotic from degradation under simulated gastric conditions. The antibiotic-loaded poly lactic-co-glycolic acid nanoparticles were able to cross an in vitro model of intestinal barrier, being more effective in the elimination of M. tuberculosis when applied against infected macrophages compared with the use of the free drug. info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/grantAgreement/ES/ISCIII/PI13-01546 info:eu-repo/grantAgreement/ES/ISCIII/PI16-01912 info:eu-repo/grantAgreement/ES/MEC/FPU14-01854 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.3 2019 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 29 / 156 = 0.186 2019 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 43 / 103 = 0.417 2019 Q2 T2 0.997 2019 Development 2019 Q1 Medicine (miscellaneous) 2019 Q1 Biomedical Engineering 2019 Q1 Materials Science (miscellaneous) 2019 Q1 Nanoscience and Nanotechnology 2019 Q2 Bioengineering 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Larrea, Ane (orcid)0000-0002-6277-7996 Rodriguez-Fernandez, Pablo Alfaro, Salvador Gracia, Begoña Universidad de Zaragoza (orcid)0000-0001-9233-5024 Lucía, Ainhoa Universidad de Zaragoza (orcid)0000-0002-0111-4697 Usón, Laura Universidad de Zaragoza (orcid)0000-0002-4678-7465 Gómez, Andromeda-Celeste Mendoza, Gracia Universidad de Zaragoza (orcid)0000-0003-2293-363X Lacoma, Alicia Domínguez, José Prat, Cristina Sebastián, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Ainsa, José Antonio Universidad de Zaragoza (orcid)0000-0003-2076-844X Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1008 X Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Proy. investigación HQA 14, 6 (2019), 707-726 Nanomedicine Nanomedicine 1743-5889 990296 http://zaguan.unizar.es/record/78020/files/texto_completo.pdf Postprint 101486 http://zaguan.unizar.es/record/78020/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78020 articulos driver 2025-03-13-08:43:49 ARTICLE