000078062 001__ 78062
000078062 005__ 20191122145056.0
000078062 0247_ $$2doi$$a10.1155/2018/8060178
000078062 0248_ $$2sideral$$a106653
000078062 037__ $$aART-2018-106653
000078062 041__ $$aeng
000078062 100__ $$aRuiz-Roldán, L.
000078062 245__ $$aPseudomonas aeruginosa Isolates from Spanish Children: Occurrence in Faecal Samples, Antimicrobial Resistance, Virulence, and Molecular Typing
000078062 260__ $$c2018
000078062 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078062 5203_ $$aPseudomonas aeruginosa is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by P. aeruginosa isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of P. aeruginosa from faecal samples of children from two different Spanish regions were characterised. Seventy-two P. aeruginosa were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 exoU-positive strains. Alteration in protein OprD showed eight different patterns. The unique imipenem-resistant strain showed a premature stop codon in OprD. Intestinal colonisation by P. aeruginosa, mainly by international clones (as ST244, ST253, and ST274), is an important factor for the systemic infections development and the environmental dissemination. Periodic active surveillance is useful to identify these community human reservoirs and to control the evolution of antibiotic resistance and virulence activity.
000078062 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/PI16-01381$$9info:eu-repo/grantAgreement/ES/ISCIII-FIS/PI12-01276
000078062 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000078062 590__ $$a2.197$$b2018
000078062 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b81 / 135 = 0.6$$c2018$$dQ3$$eT2
000078062 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b94 / 162 = 0.58$$c2018$$dQ3$$eT2
000078062 592__ $$a0.795$$b2018
000078062 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2018$$dQ2
000078062 593__ $$aMedicine (miscellaneous)$$c2018$$dQ2
000078062 593__ $$aImmunology and Microbiology (miscellaneous)$$c2018$$dQ2
000078062 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078062 700__ $$aBellés, A.
000078062 700__ $$aBueno, J.
000078062 700__ $$aAzcona-Gutiérrez, J.M.
000078062 700__ $$aRojo-Bezares, B.
000078062 700__ $$aTorres, C.
000078062 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo, F.J.$$uUniversidad de Zaragoza
000078062 700__ $$aSáenz, Y.
000078062 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, C.$$uUniversidad de Zaragoza
000078062 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000078062 773__ $$g2018, Artc ID 8060178 (2018), [8]$$pBioMed res. int.$$tBioMed Research International$$x2314-6133
000078062 8564_ $$s451478$$uhttps://zaguan.unizar.es/record/78062/files/texto_completo.pdf$$yVersión publicada
000078062 8564_ $$s34743$$uhttps://zaguan.unizar.es/record/78062/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078062 909CO $$ooai:zaguan.unizar.es:78062$$particulos$$pdriver
000078062 951__ $$a2019-11-22-14:45:55
000078062 980__ $$aARTICLE