78062 20191122145056.0 doi 10.1155/2018/8060178 sideral 106653 ART-2018-106653 eng Ruiz-Roldán, L. Pseudomonas aeruginosa Isolates from Spanish Children: Occurrence in Faecal Samples, Antimicrobial Resistance, Virulence, and Molecular Typing 2018 Access copy available to the general public Unrestricted Pseudomonas aeruginosa is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by P. aeruginosa isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of P. aeruginosa from faecal samples of children from two different Spanish regions were characterised. Seventy-two P. aeruginosa were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 exoU-positive strains. Alteration in protein OprD showed eight different patterns. The unique imipenem-resistant strain showed a premature stop codon in OprD. Intestinal colonisation by P. aeruginosa, mainly by international clones (as ST244, ST253, and ST274), is an important factor for the systemic infections development and the environmental dissemination. Periodic active surveillance is useful to identify these community human reservoirs and to control the evolution of antibiotic resistance and virulence activity. info:eu-repo/grantAgreement/ES/ISCIII/PI16-01381 info:eu-repo/grantAgreement/ES/ISCIII-FIS/PI12-01276 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.197 2018 MEDICINE, RESEARCH & EXPERIMENTAL 81 / 135 = 0.6 2018 Q3 T2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 94 / 162 = 0.58 2018 Q3 T2 0.795 2018 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2018 Q2 Medicine (miscellaneous) 2018 Q2 Immunology and Microbiology (miscellaneous) 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bellés, A. Bueno, J. Azcona-Gutiérrez, J.M. Rojo-Bezares, B. Torres, C. (orcid)0000-0002-2519-701X Castillo, F.J. Universidad de Zaragoza Sáenz, Y. (orcid)0000-0002-9742-1463 Seral, C. Universidad de Zaragoza 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 2018, Artc ID 8060178 (2018), [8] BioMed res. int. BioMed Research International 2314-6133 451478 http://zaguan.unizar.es/record/78062/files/texto_completo.pdf Versión publicada 34743 http://zaguan.unizar.es/record/78062/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78062 articulos driver 2019-11-22-14:45:55 ARTICLE