Prion protein polymorphisms associated with reduced CWD susceptibility limit peripheral PrPCWD deposition in orally infected white-tailed deer

Otero, A. ; Duque Velásquez, C. ; Johnson, C. ; Herbst, A. ; Bolea, R. (Universidad de Zaragoza) ; Badiola, J.J. (Universidad de Zaragoza) ; Aiken, J. ; McKenzie, D.

Resumen: Background
Chronic wasting disease (CWD) is a prion disease affecting members of the Cervidae family. PrPC primary structures play a key role in CWD susceptibility resulting in extended incubation periods and regulating the propagation of CWD strains. We analyzed the distribution of abnormal prion protein (PrPCWD) aggregates in brain and peripheral organs from orally inoculated white-tailed deer expressing four different PRNP genotypes: Q95G96/Q95G96 (wt/wt), S96/wt, H95/wt and H95/S96 to determine if there are substantial differences in the deposition pattern of PrPCWD between different PRNP genotypes.
Results
Although we detected differences in certain brain areas, globally, the different genotypes showed similar PrPCWD deposition patterns in the brain. However, we found that clinically affected deer expressing H95 PrPC, despite having the longest survival periods, presented less PrPCWD immunoreactivity in particular peripheral organs. In addition, no PrPCWD was detected in skeletal muscle of any of the deer.
Conclusions
Our data suggest that expression of H95-PrPC limits peripheral accumulation of PrPCWD as detected by immunohistochemistry. Conversely, infected S96/wt and wt/wt deer presented with similar PrPCWD peripheral distribution at terminal stage of disease, suggesting that the S96-PrPC allele, although delaying CWD progression, does not completely limit the peripheral accumulation of the infectious agent.

Idioma: Inglés

DOI: 10.1186/s12917-019-1794-z

Año: 2019

Publicado en: BMC VETERINARY RESEARCH 15, 50 (2019), [14]
ISSN: 1746-6148

Factor impacto JCR: 1.835 (2019)

Categ. JCR: VETERINARY SCIENCES rank: 32 / 142 = 0.225 (2019) - Q1 - T1

Factor impacto SCIMAGO: 0.787 - Veterinary (miscellaneous) (Q1) - Medicine (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/C020-2014

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)