000078165 001__ 78165
000078165 005__ 20230921135425.0
000078165 0247_ $$2doi$$a10.1021/acsomega.8b02905
000078165 0248_ $$2sideral$$a110910
000078165 037__ $$aART-2019-110910
000078165 041__ $$aeng
000078165 100__ $$aBalcells, L.
000078165 245__ $$aSPIONs' Enhancer Effect on Cell Transfection: An Unexpected Advantage for an Improved Gene Delivery System
000078165 260__ $$c2019
000078165 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078165 5203_ $$aThe clinical implantation of gene therapy is hindered by the limitations of current gene delivery vectors, namely, safety issues regarding viral vectors and low transfection efficacy regarding nonviral vectors. Thus, the design of safe and efficient gene carriers is a key point for the success of such therapies. In addition, when employing genetically modified cells for further applications, the selection of successfully modified cells becomes crucial. To address these issues, we have developed multicomponent nanoparticles composed of poly(ß-amino ester) (pBAE) polymers, plasmid DNA, and superparamagnetic iron oxide nanoparticles (SPIONs). Whereas pBAEs were initially employed as safe and biocompatible carriers with improved transfection efficiency, as compared to commercial vectors, SPIONs were used because of their magnetic character that enables cell selection. Surprisingly, the results presented here revealed an unexpected enhancer effect of SPIONs on the transfection efficiency of pBAE/pDNA polyplexes in both permissive and reluctant to transfection cell lines. This unanticipated outcome, together with the allowance to perform a selective magnetic sorting of genetically modified cells without interfering in cell transfection, opens the door to SPION-containing nanoparticles as promising tools for cell therapy approaches.
000078165 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/RTC-2015-3751-1$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-2-R
000078165 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000078165 590__ $$a2.87$$b2019
000078165 592__ $$a0.767$$b2019
000078165 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b81 / 176 = 0.46$$c2019$$dQ2$$eT2
000078165 593__ $$aChemistry (miscellaneous)$$c2019$$dQ1
000078165 593__ $$aChemical Engineering (miscellaneous)$$c2019$$dQ1
000078165 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078165 700__ $$aFornaguera, C.
000078165 700__ $$aBrugada-Vilà, P.
000078165 700__ $$aGuerra-Rebollo, M.
000078165 700__ $$aMeca-Cortés, Ó.
000078165 700__ $$0(orcid)0000-0001-7872-6301$$aMartínez, G.$$uUniversidad de Zaragoza
000078165 700__ $$aRubio, N.
000078165 700__ $$aBlanco, J.
000078165 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, J.$$uUniversidad de Zaragoza
000078165 700__ $$aCascante, A.
000078165 700__ $$aBorrós, S.
000078165 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000078165 773__ $$g4, 2 (2019), 2728-2740$$pACS Omega$$tACS OMEGA$$x2470-1343
000078165 8564_ $$s935881$$uhttps://zaguan.unizar.es/record/78165/files/texto_completo.pdf$$yVersión publicada
000078165 8564_ $$s118028$$uhttps://zaguan.unizar.es/record/78165/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078165 909CO $$ooai:zaguan.unizar.es:78165$$particulos$$pdriver
000078165 951__ $$a2023-09-21-13:29:01
000078165 980__ $$aARTICLE