78165 20230921135425.0 doi 10.1021/acsomega.8b02905 sideral 110910 ART-2019-110910 eng Balcells, L. SPIONs' Enhancer Effect on Cell Transfection: An Unexpected Advantage for an Improved Gene Delivery System 2019 Access copy available to the general public Unrestricted The clinical implantation of gene therapy is hindered by the limitations of current gene delivery vectors, namely, safety issues regarding viral vectors and low transfection efficacy regarding nonviral vectors. Thus, the design of safe and efficient gene carriers is a key point for the success of such therapies. In addition, when employing genetically modified cells for further applications, the selection of successfully modified cells becomes crucial. To address these issues, we have developed multicomponent nanoparticles composed of poly(ß-amino ester) (pBAE) polymers, plasmid DNA, and superparamagnetic iron oxide nanoparticles (SPIONs). Whereas pBAEs were initially employed as safe and biocompatible carriers with improved transfection efficiency, as compared to commercial vectors, SPIONs were used because of their magnetic character that enables cell selection. Surprisingly, the results presented here revealed an unexpected enhancer effect of SPIONs on the transfection efficiency of pBAE/pDNA polyplexes in both permissive and reluctant to transfection cell lines. This unanticipated outcome, together with the allowance to perform a selective magnetic sorting of genetically modified cells without interfering in cell transfection, opens the door to SPION-containing nanoparticles as promising tools for cell therapy approaches. info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/RTC-2015-3751-1 info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-1-R info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-2-R info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 2.87 2019 CHEMISTRY, MULTIDISCIPLINARY 81 / 176 = 0.46 2019 Q2 T2 0.767 2019 Chemistry (miscellaneous) 2019 Q1 Chemical Engineering (miscellaneous) 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Fornaguera, C. Brugada-Vilà, P. Guerra-Rebollo, M. Meca-Cortés, Ó. Martínez, G. Universidad de Zaragoza (orcid)0000-0001-7872-6301 Rubio, N. Blanco, J. Santamaría, J. Universidad de Zaragoza (orcid)0000-0002-8701-9745 Cascante, A. Borrós, S. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 4, 2 (2019), 2728-2740 ACS Omega ACS OMEGA 2470-1343 935881 http://zaguan.unizar.es/record/78165/files/texto_completo.pdf Versión publicada 118028 http://zaguan.unizar.es/record/78165/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78165 articulos driver 2023-09-21-13:29:01 ARTICLE