78180 20201116174353.0 doi 10.1371/journal.pcbi.1006691 sideral 110934 ART-2019-110934 eng (orcid)0000-0003-0389-2422 Alquézar-Baeta, C. Universidad de Zaragoza OpenCASA: A new open-source and scalable tool for sperm quality analysis 2019 Access copy available to the general public Unrestricted In the field of assisted reproductive techniques (ART), computer-assisted sperm analysis (CASA) systems have proved their utility and potential for assessing sperm quality, improving the prediction of the fertility potential of a seminal dose. Although most laboratories and scientific centers use commercial systems, in the recent years certain free and open-source alternatives have emerged that can reduce the costs that research groups have to face. However, these open-source alternatives cannot analyze sperm kinetic responses to different stimuli, such as chemotaxis, thermotaxis or rheotaxis. In addition, the programs released to date have not usually been designed to encourage the scalability and the continuity of software development. We have developed an open-source CASA software, called OpenCASA, which allows users to study three classical sperm quality parameters: motility, morphometry and membrane integrity (viability) and offers the possibility of analyzing the guided movement response of spermatozoa to different stimuli (useful for chemotaxis, thermotaxis or rheotaxis studies) or different motile cells such as bacteria, using a single software. This software has been released in a Version Control System at Github. This platform will allow researchers not only to download the software but also to be involved in and contribute to further developments. Additionally, a Google group has been created to allow the research community to interact and discuss OpenCASA. For validation of the OpenCASA software, we analysed different simulated sperm populations (for chemotaxis module) and evaluated 36 ejaculates obtained from 12 fertile rams using other sperm analysis systems (for motility, membrane integrity and morphology modules). The results were compared with those obtained by Open-CASA using the Pearson''s correlation and Bland-Altman tests, obtaining a high level of correlation in all parameters and a good agreement between the different used methods and the OpenCASA. With this work, we propose an open-source project oriented to the development of a new software application for sperm quality analysis. This proposed software will use a minimally centralized infrastructure to allow the continued development of its modules by the research community. info:eu-repo/grantAgreement/ES/DGA/A26 info:eu-repo/grantAgreement/ES/DGA-FSE/A40 info:eu-repo/grantAgreement/ES/MINECO/BES-2015-072034 info:eu-repo/grantAgreement/ES/MINECO-CICYT-FEDER/AGL2014-57863-R info:eu-repo/grantAgreement/ES/MINECO-CICYT-FEDER/AGL2017-8503-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.7 2019 2.91 2019 MATHEMATICAL & COMPUTATIONAL BIOLOGY 6 / 59 = 0.102 2019 Q1 T1 Cellular and Molecular Neuroscience 2019 Q1 BIOCHEMICAL RESEARCH METHODS 9 / 77 = 0.117 2019 Q1 T1 Computational Theory and Mathematics 2019 Q1 Ecology 2019 Q1 Molecular Biology 2019 Q1 Genetics 2019 Q1 Modeling and Simulation 2019 Q1 Ecology, Evolution, Behavior and Systematics 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-1685-6845 Gimeno-Martos, S. Universidad de Zaragoza (orcid)0000-0001-6733-9373 Miguel-Jiménez, S. Universidad de Zaragoza (orcid)0000-0001-8991-325X Santolaria, P. Universidad de Zaragoza (orcid)0000-0001-5316-1703 Yániz, J. Universidad de Zaragoza (orcid)0000-0003-1228-535X Palacín, I. Universidad de Zaragoza (orcid)0000-0003-1997-4262 Casao, A. Universidad de Zaragoza (orcid)0000-0001-9842-6197 Cebrián-Pérez, J.Á. Universidad de Zaragoza (orcid)0000-0003-1306-8517 Muiño-Blanco, T. Universidad de Zaragoza (orcid)0000-0002-2312-6402 Pérez-Pé, R. Universidad de Zaragoza 2006 440 Universidad de Zaragoza Dpto. Matemáticas Área Geometría y Topología 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 15, 1 (2019), e1006691[18 pp] PLoS Comput. Biol. PLoS computational biology 1553-734X 710004 http://zaguan.unizar.es/record/78180/files/texto_completo.pdf Versión publicada 111464 http://zaguan.unizar.es/record/78180/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78180 articulos driver 2020-11-16-17:42:25 ARTICLE