000078221 001__ 78221
000078221 005__ 20230921135427.0
000078221 0247_ $$2doi$$a10.2147/CEG.S186235
000078221 0248_ $$2sideral$$a110766
000078221 037__ $$aART-2019-110766
000078221 041__ $$aeng
000078221 100__ $$aOlbjern, C.
000078221 245__ $$aFecal microbiota profiles in treatment-naive pediatric inflammatory bowel disease - associations with disease phenotype, treatment, and outcome
000078221 260__ $$c2019
000078221 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078221 5203_ $$aPurpose: Imbalance in the microbiota, dysbiosis, has been identified in inflammatory bowel disease (IBD). We explored the fecal microbiota in pediatric patients with treatment-naive IBD, non-IBD patients with gastrointestinal symptoms and healthy children, its relation to IBD subgroups, and treatment outcomes.
Patients and methods: Fecal samples were collected from 235 children below 18 years of age. Eighty children had Crohn''s disease (CD), 27 ulcerative colitis (UC), 3 IBD unclassified, 50 were non-IBD symptomatic patients, and 75 were healthy. The bacterial abundance of 54 predefined DNA markers was measured with a 16S rRNA DNA-based test using GA-Map (TM) technology at diagnosis and after therapy in IBD patients.
Results: Bacterial abundance was similarly reduced in IBD and non-IBD patients in 51 of 54 markers compared to healthy patients (P<0.001). Only Prevotella was more abundant in patients (P<0.01). IBD patients with ileocolitis or total colitis had more Ruminococcus gnavus (P=0.02) than patients with colonic CD or left-sided UC. CD patients with upper gastrointestinal manifestations had higher Veillonella abundance (P<0.01). IBD patients (58%) who received biologic therapy had lower baseline Firmicutes and Mycoplasma hominis abundance (P<0.01) than conventionally treated. High Proteobacteria abundance was associated with stricturing/penetrating CD, surgery (P<0.01), and nonmucosal healing (P<0.03). Low Faecalibacterium prausnitzii abundance was associated with prior antibiotic therapy (P=0.001), surgery (P=0.02), and nonmucosal healing (P<0.03). After therapy, IBD patients had unchanged dysbiosis.
Conclusion: Fecal microbiota profiles differentiated IBD and non-IBD symptomatic children from healthy children, but displayed similar dysbiosis in IBD and non-IBD symptomatic patients. Pretreatment fecal microbiota profiles may be of prognostic value and aid in treatment individualization in pediatric IBD as severe dysbiosis was associated with an extensive, complicated phenotype, biologic therapy, and nonmucosal healing. The dysbiosis persisted after therapy, regardless of treatments and mucosal healing.
000078221 536__ $$9info:eu-repo/grantAgreement/EC/FP7/305676/EU/Inflammatory Bowel Disease CHARACTERization by a multi-modal integrated biomarker study/IBD-CHARACTER
000078221 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000078221 592__ $$a0.915$$b2019
000078221 593__ $$aGastroenterology$$c2019$$dQ2
000078221 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078221 700__ $$aSmastuen, M.C.
000078221 700__ $$aThiis-Evensen, E.
000078221 700__ $$aNakstad, B.
000078221 700__ $$aVatn, M.H.
000078221 700__ $$aJahnsen, J.
000078221 700__ $$aRicanek, P.
000078221 700__ $$aVatn, S.
000078221 700__ $$aMoen, A.E.F.
000078221 700__ $$aTannaes, T.M.
000078221 700__ $$aLindstrom, J.C.
000078221 700__ $$aSoderholm, J.D.
000078221 700__ $$aHalfvarson, J.
000078221 700__ $$0(orcid)0000-0003-0076-3529$$aGomollon, F.$$uUniversidad de Zaragoza
000078221 700__ $$aCasen, C.
000078221 700__ $$aKarlsson, M.K.
000078221 700__ $$aKalla, R.
000078221 700__ $$aAdams, A.T.
000078221 700__ $$aSatsangi, J.
000078221 700__ $$aPerminow, G.
000078221 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000078221 773__ $$g12 (2019), 37-49$$pClin. exp. gastroenterol.$$tCLINICAL AND EXPERIMENTAL GASTROENTEROLOGY$$x1178-7023
000078221 8564_ $$s491767$$uhttps://zaguan.unizar.es/record/78221/files/texto_completo.pdf$$yVersión publicada
000078221 8564_ $$s15743$$uhttps://zaguan.unizar.es/record/78221/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078221 909CO $$ooai:zaguan.unizar.es:78221$$particulos$$pdriver
000078221 951__ $$a2023-09-21-13:29:26
000078221 980__ $$aARTICLE