78241 20200103141021.0 doi 10.1016/j.snb.2017.09.104 sideral 102122 ART-2018-102122 eng Moya, Ana Miniaturized multiparametric flexible platform for the simultaneous monitoring of ionic: Application in real urine 2018 Access copy available to the general public Unrestricted Biomonitoring is a research topic that has largely relied on cell culture systems. Recently, the development of “Organ-on-a-Chip” (OC) platforms and the need for a continuous monitoring of these systems has increased its interest. However, the biomonitorization in these systems is still at its infancy due to the difficulty to adapt the sensors to microfluidic OC systems. In this work we have fabricated a modular, versatile, scalable, multi-analyte sensing platform, which integrates dissolved oxygen (DO), Na+, K+ and pH sensors for monitoring their concentrations in small volume of biological fluids. The platform is fabricated by means of rapid prototyping techniques using polymeric substrates, and incorporates all the necessary elements to measure the four analytes. It was designed with four gold sensing electrodes, and integrates a gold counter electrode and an Ag/AgCl pseudo-reference electrode for the electrochemical measurements in small volumes (0.83 µL) of samples. The sensors featured good sensitivities of 3.60 ± 0.2 nA (mg L-1) for DO and 69 ± 1 mV decade-1 for pH. Na+ and K+ µISE exhibited sensitivities of 57 ± 1 mV decade-1 and 52 ± 2 mV decade-1, and low limits of detection 5 × 10-6 M and 0.5 × 10-5 M respectively. This platform allows the dynamic measurement of the biological fluids parameters simultaneously, in real time and with a rapid response. The versatility of the platform allows its adaptation in any microfluidic cells culture systems. The novel multi-sensor platform has been validated in controlled buffers and with artificial urine (AU). A proof-of-concept using real mice urine (RU) has been carried out, demonstrating the good behaviour of the multi-sensing platform. info:eu-repo/grantAgreement/ES/MINECO-FEDER/DPI2015-65401-C3-3-R info:eu-repo/grantAgreement/ES/MINECO-FEDER/DPI2011-28262-C04 info:eu-repo/grantAgreement/ES/MINECO/BFU2007-62119 info:eu-repo/grantAgreement/ES/ICTS Network/MICRONANOFABS-MEINCOM This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 653626-microMole info:eu-repo/grantAgreement/EC/H2020/653626/EU/SEWAGE MONITORING SYSTEM FOR TRACKING SYNTHETIC DRUG LABORATORIES/microMole This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643694-HEARTEN info:eu-repo/grantAgreement/EC/H2020/643694/EU/A co-operative mHEALTH environment targeting adherence and management of patients suffering from Heart Failure/HEARTEN info:eu-repo/grantAgreement/ES/CSIC-IMB-CNM/ICTS NANBIOSIS-SU8 Unit-CIBER-BBN info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.393 2018 CHEMISTRY, ANALYTICAL 6 / 84 = 0.071 2018 Q1 T1 INSTRUMENTS & INSTRUMENTATION 2 / 61 = 0.033 2018 Q1 T1 ELECTROCHEMISTRY 4 / 26 = 0.154 2018 Q1 T1 1.389 2018 Condensed Matter Physics 2018 Q1 Electrical and Electronic Engineering 2018 Q1 Electronic, Optical and Magnetic Materials 2018 Q1 Surfaces, Coatings and Films 2018 Q1 Materials Chemistry 2018 Q1 Metals and Alloys 2018 Q1 Instrumentation 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Illa, Xavi (orcid)0000-0002-6043-4869 Giménez, Ignacio Universidad de Zaragoza Lazo-Fernández, Yoskaly Villa, Rosa Errachid, Abdelhamid Gabriel, Gemma 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 255, 3 (2018), 2861-2870 Sens. actuators, B, Chem. SENSORS AND ACTUATORS B-CHEMICAL 0925-4005 445161 http://zaguan.unizar.es/record/78241/files/texto_completo.pdf Postprint 101341 http://zaguan.unizar.es/record/78241/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78241 articulos driver 2020-01-03-14:01:30 ARTICLE