000078258 001__ 78258
000078258 005__ 20200813075838.0
000078258 0247_ $$2doi$$a10.1021/acs.analchem.7b03985
000078258 0248_ $$2sideral$$a103788
000078258 037__ $$aART-2018-103788
000078258 041__ $$aeng
000078258 100__ $$aSilva, C.J.
000078258 245__ $$aDetermining the Relative Susceptibility of Four Prion Protein Genotypes to Atypical Scrapie
000078258 260__ $$c2018
000078258 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078258 5203_ $$aAtypical scrapie is a sheep prion (PrPSc) disease whose epidemiology is consistent with a sporadic origin and is associated with specific polymorphisms of the normal cellular prion protein (PrPC). To determine the relative amounts of PrP polymorphisms present in atypical scrapie, total PrP was digested with chymotrypsin to generate characteristic peptides spanning relevant polymorphisms at positions 136, 141, 154, 171, and 172 of sheep PrPC. A multiple reaction monitoring method (MRM), employing 15N-labeled internal standards, was used to detect and quantify these polymorphisms present in both the PrPSc and PrPC from heterozygous (ALRRY and ALHQY or ALRQD or AFRQY) atypical scrapie-infected or uninfected control sheep. Both polymorphisms of the full length and truncated (C1) natively expressed PrPC are produced in equal amounts. The overall amount of PrPC present in the infected or uninfected animals was similar. PrPSc isolated from heterozygotes was composed of significant amounts of both PrP polymorphisms, including the ALRRY polymorphism which is highly resistant to classical scrapie. Thus, an atypical scrapie infection does not result from an overexpression of sheep PrPC. The replication of all atypical scrapie prions occurs at comparable rates, despite polymorphisms at positions 141, 154, 171, or 172.
000078258 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A17$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-65560-R
000078258 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000078258 590__ $$a6.35$$b2018
000078258 591__ $$aCHEMISTRY, ANALYTICAL$$b7 / 84 = 0.083$$c2018$$dQ1$$eT1
000078258 592__ $$a2.113$$b2018
000078258 593__ $$aAnalytical Chemistry$$c2018$$dQ1
000078258 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000078258 700__ $$aErickson-Beltran, M.L.
000078258 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza
000078258 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000078258 700__ $$aRequena, J.R.
000078258 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R.$$uUniversidad de Zaragoza
000078258 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000078258 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000078258 773__ $$g90, 2 (2018), 1255-1262$$pAnal. chem.$$tANALYTICAL CHEMISTRY$$x0003-2700
000078258 8564_ $$s260989$$uhttps://zaguan.unizar.es/record/78258/files/texto_completo.pdf$$yPostprint
000078258 8564_ $$s73712$$uhttps://zaguan.unizar.es/record/78258/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000078258 909CO $$ooai:zaguan.unizar.es:78258$$particulos$$pdriver
000078258 951__ $$a2020-08-13-07:57:56
000078258 980__ $$aARTICLE