78343 20200117221640.0 doi 10.1039/c7ja00378a sideral 105328 ART-2018-105328 eng Jiménez-Lamana, J. Detection and characterization of biogenic selenium nanoparticles in selenium-rich yeast by single particle ICPMS 2018 Access copy available to the general public Unrestricted A method based on single particle inductively coupled plasma mass spectrometry (SP-ICPMS) was developed for the analysis of commercial Se-rich yeasts, to confirm the occurrence of selenium nanoparticles in these food supplements. A considerable reduction of background levels was achieved by combining data acquisition at microsecond dwell times and the use of a H2 reaction cell, improving by a factor of 10 the current state-of-the-art methodology, and bringing size detection limits down to 18 nm for selenium nanoparticles. The presence of nanoparticulate selenium was revealed by size-exclusion chromatography ICPMS, with detection of a selenium peak at the exclusion volume of the column showing absorption at a wavelength corresponding to selenium nanoparticles. SP-ICPMS allowed us to confirm the presence of Se-nanoparticles, as well as to calculate the nanoparticle size distribution, from information about the shape and elemental composition of the nanoparticles obtained by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS), respectively. These results reveal the significance of nanoparticles in the speciation of metals and metalloids in biological samples and the capability of SP-ICPMS in combination with TEM-EDS to carry out these analyses. info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2015-68094-C2-1-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.646 2018 SPECTROSCOPY 4 / 41 = 0.098 2018 Q1 T1 CHEMISTRY, ANALYTICAL 16 / 84 = 0.19 2018 Q1 T1 0.901 2018 Spectroscopy 2018 Q1 Analytical Chemistry 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-7931-3901 Abad-Álvaro, I. Bierla, K. (orcid)0000-0002-4169-0357 Laborda, F. Universidad de Zaragoza Szpunar, J. Lobinski, R. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 33, 3 (2018), 452-460 J. anal. at. spectrom. Journal of Analytical Atomic Spectrometry 0267-9477 1065205 http://zaguan.unizar.es/record/78343/files/texto_completo.pdf Postprint 45048 http://zaguan.unizar.es/record/78343/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78343 articulos driver 2020-01-17-22:04:07 ARTICLE